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  • 1
    Electronic Resource
    Electronic Resource
    The isolation, characterization and application in the Triticeae of a set of wheat RFLP probes identifying each homoeologous chromosome arm (1989)
    Springer
    Theoretical and applied genetics 78 (1989), S. 342-348 
    Details
    ISSN: 1432-2242
    Keywords: Hexaploid wheat ; RFLPs ; Homoeology ; Chromosome arm ; Alien additions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To investigate the use of RFLP analysis in the Triticeae, a set of low copy number probes has been isolated from a wheat cDNA library. The probes identify each of the 14 homoeologous chromosome arms of wheat as determined by analysis of DNA fragments hybridizing to the probes in aneuploid lines of Chinese Spring. These probes can be used in RFLP analyses both for the assignment of homoeology of alien chromosomes or arms added to wheat, and for the determination of chromosome dosage in wheat aneuploids. Different chromosomes from various Triticeae species can therefore be followed in a wheat genetic background using a single technique. The potential uses of the set in facilitating the transfer of alien segments into wheat are outlined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00265294
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Isozyme variation and RFLPs at the β-amylase loci in wheat (1988)
    Springer
    Theoretical and applied genetics 76 (1988), S. 691-699 
    Details
    ISSN: 1432-2242
    Keywords: Hexaploid wheat ; β-Amylase ; Isozymes ; RFLPs ; IEF
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Forty-one hexaploid wheat genotypes have been examined for RFLPs detected by a β-amylase probe using three restriction enzymes, and for mature grain β-amylase isozyme polymorphism following IEF. The two homoeoallelic series assayed for RFLPs differed: little variation was found at group 2 chromosome homoeoloci, while the group 4/5 chromosome homoeoloci displayed considerable variation. Varieties that displayed a RFLP with one RE almost always did likewise with the other two REs, suggesting that most of the polymorphisms observed were due to large DNA rearrangements. Comparison of the variation in grain β-amylase isozymes with the RFLP results indicated strong associations between particular RFLP and isozyme alleles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00303514
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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