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Digitale Medien

Development of simple sequence repeats (SSRs) in olive tree (Olea europaea L.) (2000)

Rallo, P. ; Dorado, G. ; Martín, A.

Springer

Theoretical and applied genetics 101 (2000), S. 984-989

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ISSN: 1432-2242

Schlagwort(e): Key words Microsatellites ; Variable number of tandem repeats (VNTRs) ; Enriched library ; Hexamer repeat ; Null allele ; Marker-assisted selection (MAS)

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract We report the development of microsatellites or simple sequence repeats (SSRs) in the olive tree (Olea europaea L.). Forty three positive clones obtained by the screening of a GA-enriched genomic library were sequenced and primers were designed for 13 microsatellite loci. Five primer pairs amplified polymorphic products of the expected size range. SSR polymorphism was explored in a set of 46 olive cultivars. A total of 26 alleles were detected for the five loci. Heterozygosity ranged from 0.46 to 0.71. Ninety one per cent of the cultivars had unique multilocus genotypes. Microsatellite segregation was studied in a complex population from a cross between the commercial cultivars ’Leccino’ and ’Dolce Agogia’.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/s001220051571

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Digitale Medien

Chromosomal location of resistance to Septoria tritici in Hordeum chilense determined by the study of chromosomal addition and substitution lines in `Chinese Spring' wheat (2000)

Rubiales, D. ; Reader, S.M. ; Martín, A.

Springer

Euphytica 115 (2000), S. 221-224

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ISSN: 1573-5060

Schlagwort(e): Septoria tritici ; disease resistance ; Hordeum chilense ; Tritordeum