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  • 1
    Electronic Resource
    Electronic Resource
    Alginate oligosaccharides modulate cell morphology, cell proliferation and collagen expression in human skin fibroblasts in vitro (1999)
    Springer
    Archives of dermatological research 291 (1999), S. 432-436 
    Details
    ISSN: 1432-069X
    Keywords: Key words Alginate oligosaccharides ; Collagen ; Human skin fibroblasts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Effects of alginate oligosaccharides on cell proliferation and expression of collagen in cultured skin fibroblasts were studied. The oligosaccharides were found to suppress fibroblast proliferation to half the level in control cultures at a dose of 10 mg/ml during a period of 5 days. The inhibition was accompanied by a change in cell shape. The inhibition of cell proliferation was reversible, since depletion of these oligosaccharides led to a recovery of cell motility. Treatment of confluent cells with 10 mg/ml oligosaccharides for 5 days resulted in a reduction in collagen synthesis to one half of that in control cultures and inhibition of steady state levels of α1(I), α2(I), α1(III) and α1(VI) collagen mRNAs. These results suggest that alginate oligosaccharides are potential modulators of dermal fibroblasts and may provide a useful tool for the treatment of disorders related to abnormal collagen metabolism.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004030050434
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Expression of microfibril-associated glycoprotein-1 (MAGP-1) in human epidermal keratinocytes (2000)
    Springer
    Archives of dermatological research 292 (2000), S. 21-26 
    Details
    ISSN: 1432-069X
    Keywords: Key words MAGP-1 ; Elastin ; Keratinocyte ; Differentiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Microfibril-associated glycoprotein (MAGP) is a major structural component of connective tissue microfibrils. We studied the expression of MAGP-1 in cultured human keratinocytes and its modulation during Ca++-induced differentiation. RT-PCR and Western blot assays demonstrated the presence of mRNA and the polypeptide of MAGP-1 in cultured keratinocytes. MAGP-1 mRNA levels in cultured keratinocytes during Ca++-induced differentiation were enhanced eightfold with a concomitant increase in involucrin (a marker of terminal differentiation) mRNA levels. Double immunofluorescence labeling of cultured keratinocytes demonstrated that both anti-MAGP-1 and anti-involucrin antibodies reacted with the identical cells. The population of MAGP-1-producing cells in cultured keratinocytes significantly increased during Ca++-induced differentiation. These results indicate that MAGP-1 expressed by cultured keratinocytes reaches maximum levels at the stage of terminal differentiation in vitro. Double immunostaining of normal human skin with anti-MAGP-1 and anti-elastin antibodies demonstrated the colocalization of MAGP-1-positive and elastin-positive fibers in the superficial and mid-dermis. MAGP-1 produced by keratinocytes may play some functional role in the formation of dermal matrix organization in the dermis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00007456
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    Paper (German National Licenses)
    Fulltext
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