ISSN:
1432-2048
Keywords:
Diamine oxidase
;
Extracellular solution
;
Hydrogen peroxide production
;
Pisum (diamine oxidase)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Most of the diamine oxidase (EC 1.4.3.6) present in pea (Pisum sativum L. cv. Rondo) epicotyls is found in the fluid obtained by centrifuging pea epicotyl sections previously infiltrated under vacuum with a buffer solution. No detectable amount of the cytoplasmic enzyme glucose-6-phosphate dehydrogenase is present in this fluid, showing that there is very little contamination by cell contents. Polyacrylamide-gel electrophoresis and specific-activity data indicate that diamine oxidase is the most plentiful protein in the extracellular solution obtained from pea epicotyl sections and that an active process is involved in the selective transfer of the enzyme outside the cell. The possible involvement of diamine oxidase in the supply of H2O2 to peroxidase-catalyzed reactions occurring inside the cell wall is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00391430
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