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  • 1
    Electronic Resource
    Electronic Resource
    Characterization of siderophores from Ustilago maydis (1989)
    Springer
    Mycopathologia 108 (1989), S. 125-133 
    Details
    ISSN: 1573-0832
    Keywords: ferrichrome ; ferrichrome A ; hydroxamate ; siderophore and Ustilago maydis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Two siderophores, ferrichrome and ferrichrome A, were found in cultures of Ustilago maydis (DC) Corda. Both siderophores were found intracellularly and extracellularly. Their authenticity was confirmed by thin layer chromatography, HPLC, UV-visible spectrometry, paper electrophoresis, amino acid analysis, NMR and fast atom bombardment mass spectroscopy. Regulation of siderophore production by iron was examined. Repression of biosynthesis of extracellular siderophores occurred at 10−5 M iron. Ferrichrome was found intracellularly at all iron concentrations employed; in general, ferrichrome A was not found to be cell-associated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00436063
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Meiotic and mitotic stability of transforming DNA in the phytopathogenic fungus Magnaporthe grisea (1992)
    Springer
    Current genetics 21 (1992), S. 55-60 
    Details
    ISSN: 1432-0983
    Keywords: Transformation ; Stability ; Hygromycin B resistance ; Rice blast fungus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Magnaporthe grisea was transformed with cosmid pAN7-2 encoding hygromycin B resistance but containing no homology with the M. grisea genome. Rearrangement of the integrated DNA was detected in several hygromycin B-resistant progeny from cross Guy11-T10-1 (a single-copy integration site transformant)x2539 (sensitive wild-type parent), but not in hygromycin B-resistant progeny from four other crosses. Transformants produced typical lesions when inoculated onto host plants. Southern hybridization revealed rearrangements of integrated DNA in single conidial isolates of highcopy transformant 2539-T1-1 re-isolated from host plants, characterized by excision of one or more copies of the transforming plasmid. Plasmid loss and rearrangement were also observed within single conidial isolates derived from transformant 2539-T1-1 following ten asexual generations on non-selective agar medium. These examples of instability of integrated DNA in M. grisea transformants suggest that caution should be exercised in the use of transformation for assessing the phenotypic effects of specific introduced genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00318655
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    Paper (German National Licenses)
    Fulltext
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