ISSN:
1573-2568
Keywords:
INTERLEUKIN-1β
;
GASTRIC EPITHELIAL CELL
;
GROWTH FACTOR
;
WOUND REPAIR
;
RGM1
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract We examined the effect of interleukin-1β(IL-1β) on spontaneous and enhanced restoration(cell migration and proliferation) using an in vitrowound model comprising a confluent monolayer of ratgastric epithelial RGM1 cells. Repair of an artificialwound in a cell monolayer was found to be time- andconcentration-dependent when the cells were incubatedwith epidermal growth factor (EGF) or transforming growth factor (TGF)-α alone for up to 24hr. The growth factors also stimulated DNA synthesissignificantly for 24 hr in a concentration-relatedmanner. IL-1β had no effect on wound restoration in the absence of the growth factors. However, itmarkedly inhibited the restoration enhanced by EGF andTGF-α, the inhibition being about 60% and 70%,respectively. In addition, IL-1β significantly reduced the DNA synthesis stimulated by thegrowth factors. The EGF- and TGF-α-enhancedrestoration was reduced by about 30% by mitomycin C,which potently inhibited the stimulated DNA synthesis.Mitomycin C had no effect on the spontaneous restoration.Even when treated with mitomycin C, the inhibitoryeffect of IL-1β on the enhanced wound repair wasstill observed; however, the extent of the inhibition was decreased. These results indicate thatIL-1β inhibits the migration as well as theproliferation of gastric epithelial cells enhanced byEGF and TGF-α, resulting in a failure of woundhealing.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1018890419648
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