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  • Digitale Medien  (2)
  • IS1  (1)
  • Saintpaulia ionantha  (1)
  • 1
    Digitale Medien
    Digitale Medien
    IS1-mediated mobility of the aerobactin system of pColV-K30 in Escherichia coli (1988)
    Springer
    Molecular genetics and genomics 213 (1988), S. 487-490 
    Details
    ISSN: 1617-4623
    Schlagwort(e): pColV ; Aerobactin ; Transposition ; IS1 ; Cointegration
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Genes determining the high affinity iron transport system mediated by the siderophore aerobactin are flanked in the enterobacterial plasmid pColV-K30 by inverted repeats of IS1 sequences, suggesting that the aerobactin genes are part of a transposon. To study this possibility, the entire region between the two IS1 sequences was cloned as an 18 kb HindIII-BamHI restriction fragment in pUC8 giving plasmid pMO1. A number of derivatives of pMO1, in which aerobactin genes were tagged with a kanamycin resistance gene, were prepared in order to assess the ability of both IS1s to promote the formation of cointegrates with pCJ105, an F derivative devoid of insertion sequences. Mating-out assays indicated that both flanking IS1s were active in cointegrate formation at detectable frequencies. In some cases, the cointegrates could be resolved, the final result being a transposition-like event for the entire aerobactin system.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00339620
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    The requirement of chrysobactin dependent iron transport for virulence incited by Erwinia chrysanthemi on Saintpaulia ionantha (1991)
    Springer
    Plant and soil 130 (1991), S. 263-271 
    Details
    ISSN: 1573-5036
    Schlagwort(e): bacteria ; Erwinia chrysanthemi ; iron transport ; phytopathogenicity ; Saintpaulia ionantha ; virulence
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract To incite a systemic disease on its specific host, Saintpaulia ionantha, the soft-rot Erwinia chrysanthemi strain 3937 requires a functional high affinity iron transport system. Under iron starvation, strain 3937 produces chrysobactin, a novel catechol-type siderophore. Recent advances in the biochemistry and genetics of iron assimilation in E. chrysanthemi are reported. Analysis of leaf intercellular fluid from healthy and infected plants suggests: (i) leaf vessels in which the bacteria develop during infection would be low in free iron and (ii) chrysobactin could be produced in planta.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00011882
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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