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  • 1
    ISSN: 1437-160X
    Keywords: Cryoprecipitates ; SLE ; Complement ; Antibody ; Rheumatoid factor ; DNA ; Immune complexes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Artificial cryoprecipitates have been prepared from SLE sera by incubation of anti-double stranded DNA with 3H-PM2 DNA, IgM rheumatoid factor, and complement to study further the relationships between natural cryoprecipitation and the DNA/anti-DNA system in this disorder. Addition of rheumatoid factor and/or complement to a mixture of SLE serum and 3H-DNA results in increased precipitation of the 3H-DNA following incubation at 4 °C. Precipitations formed with added complement or complement plus rheumatoid factor could be rapidly resolubilized or reprecipitated by warming to 37 °C or recooling to 4 °C, i.e., exhibited the behavior of natural cryoprecipitates. The resolubilized cryoprecipitates contained excess antibody, because they bound significant quantities of additional 3H-DNA in the Farr assay. Of special interest was the observation that DNA binding activity frequently could be recovered from resolubilized cryoprecipitates even though 3H-DNA was not added during the original cryoprecipitate preparation. With certain cryoprecipitations prepared in this way, the DNA binding activity was further increased by deoxyribonuclease treatment, raising the possibility that endogenous DNA may be a constituent of such cryoprecipitates. The implications of these findings with respect to the nature of antibody-DNA immune complexes in SLE are discussed.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    European journal of applied physiology 50 (1983), S. 319-329 
    ISSN: 1439-6327
    Keywords: Maximum aerobic capacity ; Dietary-induced thermogenesis ; Body fat ; Resting metabolic rate ; Luxuskonsumption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Resting metabolic rate (RMR), dietary thermogenesis (DIT), aerobic capacity ( $$\dot V$$ O2 max), and percent body fat were measured in a group of sixteen women and ten men. DIT was significantly positively correlated with aerobic capacity (r=0.658). Percent body fat was significantly negatively correlated with $$\dot V$$ O2 max (r=−0.727). RMR was independent of $$\dot V$$ O2 max, or body fatness, but women had a significantly higher average RMR than men. Three men and three women participated in an endurance training program which significantly increased $$\dot V$$ O2 max. DIT was increased in proportion to the increase in aerobic capacity (r s=0.924), RMR was unchanged, and body fat decreased in males but not females. The controversy surrounding “luxuskonsumption” may stem in part from not taking into account individual differences in aerobic capacity when measuring DIT.
    Type of Medium: Electronic Resource
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