ISSN:
1434-0879
Keywords:
Prostatic acid phosphatase
;
Lysosomes
;
Prostatic antigen
;
Immunoelectron microscopy
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The ultrastructural localization of secretory prostatic acid phosphatase (PAP) in human benign prostate tissue was accomplished using the immunogold technique on ultrathin Lowicryl sections. Polyclonal antibodies directed against secretory PAP (MW 50 kD) and the lysosomal enzymes α-glucosidase and β-galactosidase as well as an antiserum dircted against prostatic antigen (PA) were used. PAP was found in secretory vacuoles of columnar secretory epithelial cells. In addition, double labeling experiments revealed that secretory PAP was also localized in electrondense organelles of columnar epithelial cells containing α-glucosidase and β-galactosidase. PA was exclusively found in secretory vacuoles of columnar secretory epithelial cells. The results demonstrate the presence of secretory PAP within functional lysosomes and secretory vacuoles of the prostatic columnar epithelial cells and the absence of such PAP-containing lysosomes in the basal cells of the prostatic acini.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00262386
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