ISSN:
1434-4726
Keywords:
Superoxide radicals
;
Manganese superoxide dismutase
;
Immunohistochemical method
;
Cochlear labyrinth
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract There has been recent increasing interest in the involvement of superoxide radicals (O2 −) and their scavenging enzymes, the superoxide dismutases, in the pathophysiology of certain diseases. Since mitochondria are significant intracellular sources of O2 − and important targets of oxidant injury, determining the intracochlear localization of mitochondrial O2 − scavenging enzyme may provide important insight into the pathogenesis of injury due to cochlear oxidants. In order to locate the mitochondrial O2 − scavenging enzyme, manganese superoxide dismutase (MnSOD), the authors used a modified immunoglobulin peroxidase bridge sequence method to detect MnSOD in paraffin-embedded, formalin-fixed rat cochleas. Site-specific immunolocalization of MnSOD could be demonstrated in the cochlear labyrinth, suggesting that the generation of intracochlear O2 − was possibly implicated in the metabolically active sites and sites rich in vascularity. This study also provided a useful probe for detecting MnSOD immunohistochemically from ethylenediamine tetraacetic acid-treated materials without requiring an antigen retrieval procedure.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00171142
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