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  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Cell & tissue research 155 (1974), S. 513-523 
    ISSN: 1432-0878
    Schlagwort(e): Dentinogenesis ; Ca45 ; Autoradiography, Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Beschreibung / Inhaltsverzeichnis: Résumé Le trasfert du Ca45 à travers la papille dentaire et la couche des ondontoblastes, vers la prédentine et la dentine, a été étudié par autoradiographie quantitative en microscopie électronique, au niveau de germes dentaires de chats nouveau-nés, après injection intraveineuse. Deux voies de transfert, d'importance sensiblement égale, ont pu être mises en évidence. La voie directe emprunte, à partir de la lumière des capillaires, les espaces intercellulaires de la papille dentaire et des odontoblastes, pour atteindre la dentine via la prédentine. La seconde voie consiste, après passage par les espaces extracellulaires de la papille dentaire, en un transfert intracellulaire par l'odontoblaste. Parmi les organelles, ce sont les mitochondries, puis l'appareil de Golgi qui sont progressivement les plus marqués à 5 min., 30 min. et 1 h. après injection intraveineuse. Le calcium diffuse dans le cytoplasme des prolongements odontoblastiques sans être associé aux vésicules denses allongés. A 6 heures, le maximum de radioactivité est observé dans la dentine intercanaliculaire et en particulier, au voisinage de la jonction émail-dentine.
    Notizen: Summary Calcium transfer from the capillaries of the dental papilla to predentine and dentine has been studied by quantitative electron microscopic autoradiography in toothgerms of newborn cats following intravenous injection of Ca45. Two transfer pathways of almost identical importance were demonstrated. The direct pathway from the capillaries followed the intercellular spaces of the dental papilla and between the odontoblasts to reach dentine via predentine. The second route involved passage through the intercellular spaces of the dental papilla, and then a transcellular transfer through the odontoblast. Of the odontoblast organelles, the mitochondria and the Golgi apparatus were most heavily and progressively labelled at 5 min, 30 min. and 1 h. after intravenous injection. The calcium diffused throughout the cytoplasm of the odontoblast process without being associated with the dense elongated vesicles. At 6 hours, the maximum radioactivity was observed in the intertubular dentine, especially along the enamel-dentine junction.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    International journal of clinical oncology 4 (1999), S. 343-347 
    ISSN: 1437-7772
    Schlagwort(e): Key words KAI1, Ovarian tumors ; PCR ; Immunohistochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Background. KAI1 is a potential metastasis suppressor gene for prostate cancer. Decreased expression of KAI1 mRNA has been shown to be associated with the formation of metastasis and the progression of prostate, lung, breast, pancreatic, and bladder cancer. It has also been reported, however, that KAI1 expression is unchanged in metastatic and nonmetastatic esophageal and gastric cancer. We performed the present study to investigate the function of KAI1 in the progression and/or metastasis of ovarian cancer. Methods. We investigated the mRNA expression levels of the KAI1 gene, using quantitative polymerase chain reaction (PCR), in 29 ovarian tumors (1 adenoma, 2 low- malignant potential tumors, 9 adenocarcinomas without metastasis, and 17 adenocarcinomas with metastasis), seven ovarian cancer cell lines, and two normal ovaries. Using a thermal cycler, we found that the KAI1 gene was amplified in parallel with an internal control gene, β-Actin. The relative expression ratio (KAI1/β-Actin) as measured by densitometry was used to evaluate gene expression. Immunohistochemical localization of the KAI1 protein in ovarian cancer tissues was confirmed by the avidin-biotin peroxidase complex (ABC) method. Results. The mRNA expression levels of KAI1 were consistent in normal ovary, ovarian tumor samples, and ovarian cancer cell lines. No statistically significant difference in the KAI1 mRNA expression level was found in ovarian cancer samples with or without metastasis. Immunohistochemistry revealed that the KAI1 protein was expressed in the cell membranes of ovarian cancer cells. Conclusions. Our results suggest that reductions in KAI1 mRNA expression are not involved in either the progression or metastasis of ovarian carcinomas.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    ISSN: 0021-9304
    Schlagwort(e): BMP ; geometry ; carriers ; hydroxyapatite ; Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin , Technik allgemein
    Notizen: Bone morphogenetic protein (BMP) is known to require a suitable carrier to induce ectopic bone formation in vivo. Hydroxyapatite ceramics have been reported to be effective in some forms but ineffective in others as a carrier of BMP-induced bone formation. In this study we compare three geometrically different forms of hydroxyapatite to examine their functions as carriers of BMP-induced bone formation. A fraction containing all the active BMPs (BMP cocktail) was partially purified from a 4M guanidine extract from bovine bone by a three-step chromatographic procedure. The BMP cocktail was combined with each of three forms of hydroxyapatite - solid particles (SPHAP), porous particles (PPHAP), and coral-replicated porous tablets (coral-HAP) - and implanted subcutaneously into rats. Both the PPHAP and coral-HAP systems induced osteogenesis 2 weeks after implantation, as evidenced by morphological and biochemical observations. Details of the osteogenetic process were followed by double-fluorescence labeling in the coral-HAP system to confirm bone formation on the surface of hydroxyapatite. However, there was no evidence of osteogenesis or chondrogenesis in the SPHAP system. The results indicate that the geometry of the interconnected porous structure in PPHAP and coral-HAP create spaces for vasculature that lead to osteogenesis while the smooth structure and close contact of particles in SPHAP inhibit vascular formation and proliferation of mesenchymal cells, preventing bone and cartilage formation. It was concluded that the geometrical structure in hydroxyapatite ceramics that induces vasculature is crucial as a carrier for BMP-induced bone formation. © 1998 John Wiley & Sons, Inc. J Biomed Mater Res, 39, 190-199, 1998.
    Zusätzliches Material: 8 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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