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  • Electronic Resource  (4)
  • In situ hybridization  (2)
  • Interspecific hybridization  (2)
  • 1
    ISSN: 1432-041X
    Keywords: Chymotrypsin ; Larva ; Metamorphosis Mollusc ; In situ hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In the non-feeding larva of the marine gastropod, Haliotis rufescens, gut morphogenesis is initiated at metamorphosis. Intestine-specific chymotrypsin gene expression begins in amoebocytes located in the dorsoposterior region of the undifferentiated digestive gland prior to metamorphosis, 5 d post-fertilization. Transcript accumulates steadily in these cells over the next 6 d while the amoebocytes migrate slowly dorsally. Induction of metamorphosis dramatically accelerates the rates of chymotrypsin mRNA accumulation and amoebocyte migration, and is required for homing of the amoebocytes to the hindgut region. Induction of chymotrypsin gene expression occurs only in larvae that had developed competence to recognize an exogenous morphogenetic cue and initiate metamorphosis, with a more pronounced increase in chymotrypsin mRNA accumulation in occurring older larvae. Chymotrypsin mRNA accumulation patterns suggest that hindgut cell specification occurs prior to metamorphosis, but that completion of the morphogenetic program requires signaling events associated with metamorphosis.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-041X
    Keywords: Key words Ascidian ; Serine protease ; Differential display ; Gene expression ; In situ hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We have studied gene expression during ascidian embryonic development using the technique of differential display and isolated partial cDNA sequences of 12 genes. Developmental regulation of these genes has been confirmed by northern hybridization analysis. Further cDNA cloning and sequence analysis of an mRNA that is present during gastrulation, neurulation and tailbud formation reveals that it encodes a novel serine protease containing a single kringle motif and catalytic domain. The spatial expression of this gene, designated Hmserp1, is restricted to precursor cells of the epidermis. The structure and expression of Hmserp1 is discussed in relation to possible functions during development.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2242
    Keywords: Tetraploid triticale ; Chromosome pairing ; Interspecific hybridization ; Genome re-arrangement ; Genome affinity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two F5 strains of tetraploid triticale (2n= 4x=28), obtained from 6x triticaleX2 rye progenies, were crossed with diploid and tetraploid rye, some durum and bread wheats, and various 8x and 6x triticale lines. Meiosis in the different hybrid combinations was studied. The results showed that the haploid complement of these triticales consists of seven chromosomes from rye and seven chromosomes from wheat. High frequencies of PMCs showing trivalents were observed in hybrids involving the reference genotypes of wheat and triticale. These findings proved that several chromosomes from the wheat component have chromosome segments coming from two parental wheat chromosomes. The origin of these heterogeneous chromosomes probably lies in homoeologous pairing occurring at meiosis in the 6x triticaleX2x rye hybrids from which 4x triticale lines were isolated. A comparison among different hybrids combinations indicated that the involvement of D-genome chromosomes in homoeologous pairing is quite limited. In contrast, meiotic patterns in 4x triticale X 2x rye hybrids showed a quite high pairing frequency between some R chromosomes and their A and B homoeologues.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 79 (1990), S. 646-653 
    ISSN: 1432-2242
    Keywords: Triticale ; Interspecific hybridization ; Genome rearrangement ; Biochemical markers ; Electrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Fifty-two progenies originating from a cross between 8x and 4x triticale were submitted to cytogenetic analyses and to various electrophoretic studies [high-molecular-weight (HMW) glutelins, HMW secalins, γ-secalins, α and ω-gliadins, β-amylases] to identify new genetic structures, more specifically the input of the D genome in a genetic context other than the wheat one. Markers of the rye genome (HMW and ω-secalins) were identified in all of the triticale lines, but they originated either from the 4x or from the 8x parent, or from both. Chromosomes 4A, 1B, and 2R, present in both parents, showed the same banding patterns in all progenies. Chromosomes 1R and 5R, present in both parents, showed heterogeneous labelling. The expression of chromosomes 6A, 1D, and 4D, present in the 8x parent only, was more complex with a possible involvement of a regulatory system. Several hexaploid progenies had introgressed part of the D genome, suggesting that crossing 8x and 4x triticale was a practicable approach for transferring D chromosomes into hexaploid triticale.
    Type of Medium: Electronic Resource
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