ISSN:
1434-4726
Keywords:
Adenylate energy charge
;
Energy status
;
Phosphorylation state
;
Inner ear perfusion
;
Artificial blood
;
Fluorocarbons
;
Endolymphatic potential
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary The levels of the adenine nucleotides ATP, ADP, and AMP in the stria vascularis were measured under normal conditions, and following various durations of ischemia. The concentrations of these compounds were used for the calculation of the adenylate energy charge, the energy status and the phosphorylation state of the stria. Following 10 min of ischemia the adenylate energy charge had decreased three fold, the energy status seven fold and the phosphorylation state 14 fold. To study the potential for recovery of strial function following various brief and prolonged ischemic intervals, a method for the perfusion of the ear via the anterior inferior cerebellar artery was developed. For various reasons it was found advantageous to use “artificial blood” as perfusate, relying upon fluorocarbons as oxygen carriers. The endolymphatic potential was used as electrical indicator of strial function. Recovery of the endolymphatic potential following brief periods of ischemia was paralleled by a corresponding increase of the ATP levels and a drastic decrease of the AMP levels of the stria vascularis. Preliminary results on the effects of substrate-free perfusion are presented.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00455229
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