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  • 1
    ISSN: 1432-0533
    Keywords: Key words Spinal ventral horn ; Aging ; Interneuron ; Alpha motor neuron ; Morphometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A cytoarchitectonic study of spinal ventral horn cells was performed to identify age-related changes. The diameter distribution of ventral horn neurons of the fourth lumbar segment of the spinal cord and their size and topographical distributions were investigated in 14 autopsy cases. These cases represented patients of 18–100 years of age who had died of non-neurological diseases. The results indicate that small neurons widely distributed in the intermediate zone of the ventral horn significantly diminished with aging (P 〈 0.0005, r = –0.898), whereas medium-sized and large neurons located in the medial and lateral nuclei showed only a slight decrease with advancing age. The total number of neurons in the whole ventral horn was also noted to decrease significantly with aging (P 〈 0.0005, r = –0.899). While small neurons in the intermediate zone of the ventral horn are thought to be mostly interneurons, their physiological function still remains obscure in many respects. The findings of this study provide insight into age-related cell loss in terms of size and location.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0533
    Keywords: Key words X-linked recessive spinal and bulbar ; muscular atrophy ; Corticospinal tract ; Spinal ventral horn cells ; Alpha motor neuron ; Interneuron
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A quantitative study was performed on spinal cord lesions in seven patients with X-linked recessive spinal and bulbar muscular atrophy. The myelinated fiber density of the lateral corticospinal tracts at the T7 cord level was well preserved for both large and small myelinated fibers. On the other hand, neurons in the L4 ventral horn were markedly depleted; marked loss was noted of the large alpha and medium-sized gamma motor neurons located in the lateral and medial nuclei as well as the small neurons in the intermediate zones of the ventral horn. These results suggest that myelinated fiber density and fiber-size distribution in the corticospinal tract are well preserved and that neuronal loss in the ventral horns is not restricted to alpha and gamma motoneurons but also involves small interneurons.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1615-6102
    Keywords: Lilium ; Pollen tube ; Proton-induced X-ray emission (PIXE) ; The Heidelberg proton microprobe ; Polar zinc distribution ; mRNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The zinc distribution in pollen tubes ofLilium longiflorum was analysed by proton-induced X-ray emission (PIXE) with the Heidelberg proton microprobe. A very high zinc concentration was measured in the pollen tube tip region.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1615-6102
    Keywords: Elemental map ; Lilium ; Oxford proton microprobe ; Scanning proton microprobe ; Proton induced X-ray emission ; Pollentube
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Pollen tubes ofLilium longiflorum were fixed with glutaraldehyde and investigated unsectioned with the Oxford scanning proton microprobe (SPM). Two-dimensional maps which show the distribution and concentration of phosphorus, sulphur, chlorine, potassium, calcium, iron, copper, zinc and arsenic are presented. The maps show that, within the pollen tube tip region, calcium and zinc exhibit relatively steep longitudinal concentration gradients compared to the more flat distributions of phosphorus and sulphur. Chlorine, potassium, iron and copper appear equally distributed along the tube. All elements with the expception of arsenic show the highest concentration within the cell protoplasm and not in the cell wall. Additional signals of arsenic, chlorine and potassium originate from the remaining fixative dried around the tube, containing also the free ions of the cell. The arsenic signals originate exclusively from the buffer used during fixation. The different maps are compared and discussed in relation to their significance to the pollen tubes.
    Type of Medium: Electronic Resource
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