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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Der Chirurg 69 (1998), S. 443-449 
    ISSN: 1433-0385
    Keywords: Key words: Diverticular disease ; Emergency operation ; Prognostic factors ; Morbidity ; Mortality. ; Schlüsselwörter: Dickdarmdiverticulitis ; Notfalloperation ; Prognosefaktoren ; Morbidität ; Letalität.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung. In einer retrospektiven Studie wurden die Prognosefaktoren bzgl. Morbidität und Letalität von 105 notfallmäßig operierten Patienten mit perforierter Dickdarmdiverticulitis untersucht. Bei unterschiedlichem Ausprägungsgrad der Diverticulitis (Hinchey-Klassifikation I: 8,6 %, II: 14,3 %, III: 57,1 %, IV: 20,0 %) betrug die Letalität 12,4 %. In der multivariaten Analyse waren die präoperative Sepsis mit Leukopenie und Leberfunktionsstörungen, kardiale Risikofaktoren und die Adipositas eigenständige prognoseverschlechternde Parameter. Bei gleichzeitig vorliegender Immunsuppression kam es zu einem deutlichen Anstieg der Letalität auf 33 %. Das Ausmaß der Peritonitis zeigte keinen Einfluß auf die Prognose. Das Operationsverfahren (Kontinuitätsresektion 12,4 %, Diskontinuitätsresektion 61,9 %, nicht resezierende Verfahren 25,7 %) hatte multivariat nur einen Einfluß auf die vermehrte Ausbildung allgemeiner Komplikationen.
    Notes: Summary. Morbidity and mortality after emergency procedures in 105 patients with perforated colonic diverticulitis were evaluated in a retrospective study. In different stages of diverticulitis (Hinchey classification: I, 8.6 %; II, 14.3 %; III, 57.1 %; IV, 20.0 %) mortality was 12.4 %. Preoperative sepsis syndrome with leucopenia and disturbed liver function, cardiac risk factors and obesity were independent prognostic factors in multiple logistic regression. Accompanied by immunosuppression the mortality rate remarkably increased to 33 %. The stage of peritonitis showed no influence on the prognosis. In multivariate analysis, surgical procedure (primary resection 12.4 %, Hartmann's procedure 61.9 %, non-resection procedures 25.7 %) showed influence only on increased general complications.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Pflügers Archiv 437 (1999), S. 517-522 
    ISSN: 1432-2013
    Keywords: Key words Electronic autoradiography ; Enzyme heterogeneity ; α Isoforms ; Isoform-specific antibodies ; Na+/K+-ATPase ; [3H]Ouabain binding ; Shark rectal gland ; Squalus acanthias
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract  Purified Na+/K+-ATPase (EC 3.6.1.37) isolated from the rectal gland of Squalus acanthias was characterized in ouabain-binding studies and with respect to isoform(s) of the α peptide. To avoid enzyme inactivation [3H]ouabain equilibrium binding was carried out at 20°C. The heterogeneity of Na+/K+-ATPase isolated from shark rectal gland was similar in [3H]ouabain binding as previously seen in hydrolytic studies. The binding isotherms were compatible with the existence of a high-affinity (K dis 0.69 nM) and a low-affinity (K dis 42 nM) component of 1.46 and 0.79 nmol.(mg protein)–1, respectively. In Western blots the α peptide of the enzyme hybridized with an isoform-specific polyclonal antibody raised to an α3-specific region of the large intracellular domain of rat Na+/K+-ATPase, but not with the supposed α3-specific monoclonal antibody MA3-915 with its epitope near the N-terminus. Semi-quantitative analysis of the reaction of the α3-specific polyclonal antibody with the α peptide from the shark enzyme compared to the reaction with α peptide from rat brain enzyme indicated that this region is not exactly the same in the two species. The α peptide of shark enzyme was not recognized by α1- or α2-specific polyclonal antibodies, or by the α1-specific monoclonal antibodies 3B and F6. The large intracellular domain of Na+/K+-ATPase from shark rectal gland thus seems to be α3-like and no α isoform heterogeneity seems able to account for the heterogeneity seen in ouabain binding.
    Type of Medium: Electronic Resource
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