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  • 1
    Electronic Resource
    Electronic Resource
    Transport properties of a C. albicans amino-acid permease whose putative gene was cloned and expressed in S. cerevisiae (1993)
    Springer
    Current genetics 24 (1993), S. 487-490 
    Details
    ISSN: 1432-0983
    Keywords: Amino-acid permease ; Heterologous complementation ; Candida albicans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Using a gene bank of C. albicans, the lysine-permease deficiency in a strain of S. cerevisiae was complemented, and the restriction map of the corresponding C. albicans DNA fragment was constructed. Its expression in S. cerevisiae showed that the permease of C. albicans actively transports arginine (KT=18 μmol/l, Jmax=26 nmol/min per mg dry weight), lysine (KT=12 μmol/l, Jmax=18 nmol/min per mg dry weight), histidine (KT=37 μmol/l, Jmax=9.7 nmol/min per mg dry weight), as well as their toxic analogues canavanine and thialysine, with high affinity. The intracellular concentration of basic amino acids transported into S. cerevisiae by the C. albicans permease reaches more than a thousand-times-higher value compared to the external concentration in the medium. Accumulated amino acids do not leave the cells. The uptake is strongly reduced by the protonophores and inhibitors of plasma membrane H+-ATPase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00351710
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Genetic diversity among wild and cultivated populations of Hevea brasiliensis assessed by nuclear RFLP analysis (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 199-207 
    Details
    ISSN: 1432-2242
    Keywords: Hevea brasiliensis ; RFLP ; Nuclear probes ; Isozyme ; Genetic diversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Restriction fragment length polymorphism was assessed in wild and cultivated populations of Hevea brasiliensis using random probes from an Hevea nuclear library. One-hundred-and-sixty-four individuals were surveyed, and the results discussed in the light of previous work performed on isozyme variation. Both studies show that germplasm collections have led to an effective enrichment of the genetic resources available for Hevea breeding, and that cultivated clones have conserved a relatively high level of polymorphism, despite their narrow genetic base and their high level of inbreeding. An equivalent level of polymorphism is revealed by random nuclear probes and isozymes. However, the genetic structuring of the diversity appears more striking using RFLP markers. Wild accessions can be divided into three genetic groups according to their geographical origin. The present results are an essential guide to the incorporation of wild material in breeding schemes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225898
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    Paper (German National Licenses)
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