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Digitale Medien

Determination of Hα,Hβ and Hβ,C′ coupling constants in13C-labeled proteins (1992)

Eggenberger, U. ; Karimi-Nejad, Y. ; Thüring, H. ; [weitere]

Springer

Journal of biomolecular NMR 2 (1992), S. 583-590

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Details

ISSN: 1573-5001

Schlagwort(e): 3D NMR ; J coupling constants ; Isotopic labeling ; Protein structure ; χ1 angle ; SOFT-HCCH-COSY ; SOFT-HCCH-E.COSY ; Selective heteronuclear decoupling

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Chemie und Pharmazie

Notizen: Summary A sensitive method to assign Hβ protons stereospecifically as well as to determine rotamer populations about χ1, in two 3D experiments is presented. The SOFT-HCCH-COSY experiment allowed us to measure the3J(Hβ,C′) couplings, using constant time evolution of Cα in t2 and Caliphatic-selective decoupling during t3. The SOFT-HCCH-E.COSY experiment allowed us to measure the3J(Hα,Hβ) couplings, using constant time evolution of Cα in t2, a small flip angle1H excitation pulse in the second mixing time, and double-band-selective decoupling (aliphatic and carbonyl carbons) during t3. The method was applied to ribonuclease T1.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF02192847

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