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  • 1995-1999  (2)
  • Key words: Clara cell 10-kDa protein—Asthma—Urinary protein 1.  (1)
  • Marine brown alga  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Protoplast isolation in the marine brown alga Dictyopteris prolifera (Dictyotales) (1995)
    Springer
    Plant cell reports 14 (1995), S. 571-574 
    Details
    ISSN: 1432-203X
    Keywords: Crassostrea gigas ; Dictyopteris prolifera ; Marine brown alga ; Protoplast ; Single cell
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Protoplasts were isolated from thalli of Dictyopteris prolifera using a mixture of crude enzymes from vicera of live oysters (Crassostrea gigas) and the following commercial enzymes: an abalone enzyme, cellulase, polygalacturonase and hemicellulase. The enzyme mixtures produced up to 3.3 × 107 cells per l g of tissue fresh weight. The conversion to protoplasts of the cells was about 100% using the oyster enzyme or the abalone enzyme alone. The optimum pH for protoplast isolation was 6.0 and 20 hours were required for conversion to protoplasts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00231940
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Serum Levels of Clara Cell 10-kDa Protein Are Decreased in Patients with Asthma (1999)
    Springer
    Lung 177 (1999), S. 45-52 
    Details
    ISSN: 1432-1750
    Keywords: Key words: Clara cell 10-kDa protein—Asthma—Urinary protein 1.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract. Clara cell 10-kDa protein (CC10), the predominant product from nonciliated cells in the epithelial lining of bronchioles (Clara cells), has been shown to have immunomodulatory and antiinflammatory activity and may play a role in controlling airway inflammation. This study was designed to measure serum CC10 concentrations in healthy and asthmatic nonsmokers. Serum CC10 concentrations in asthmatic nonsmokers were significantly lower than in healthy nonsmokers. Asthmatic patients with a long duration of the disease (≥10 years) had significantly lower serum CC10 levels than those with a short duration of the disease (〈10 years). There was no significant difference in serum CC10 levels in asthmatic patients between the time of the asthmatic attack and the stable condition. Serum CC10 levels may reflect decreased production of CC10 caused by remodeling of the small airways in asthma.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/PL00007626
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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