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Notizen: Abstract Methods: In order to evaluate the stress and immunological response to laparoscopic and conventional colon resection we operated on male Wistar rats (350–380 g), performing either laparoscopic (n= 15) or open colon resection (n= 15). A third group (n= 10) underwent anesthesia only. Immediately before and after surgery as well as 1 and 7 days postoperatively a 1 ml sample of blood was taken from the retrobulbar veinous plexus. Stress (corticosterone) and immune parameters (neopterin and interleukin [IL] 1-β) were measured. Furthermore, the body weight as a parameter of postoperative recovery was monitored. Results: The analysis of variance showed significant differences between the three groups over a period of 1 week (p 〈 0.0001 for corticosterone, p= 0.0854 for IL 1-β, p= 0.0045 for neopterin). Additionally in a t-test significant differences were found between the laparoscopic and conventional group with regard to corticosterone (p= 0.08), to neopterin (p= 0.045), and to IL 1-β (p= 0.0043) at the end of the operation. One week after the operation the stress and immune parameters were back to normal levels in each group except IL 1-β, but the recovery indicated by body weight was different according to the kind of the applied operative procedure: 7 days postoperatively the rats lost 5.99% of their body weight after open surgery and only 2.4% after laparoscopic surgery. After anesthesia only the body weight increased by about 4.8%. Conclusion: Laparoscopic colon resection alters the stress and immune system of healthy rats less than open colon resection. This observation is confirmed by the quicker recovery in laparoscopically operated rats.

Notizen: Abstract Background: We designed a study to evaluate the short- and long-term outcome of laparoscopic vs conventional colonic resection in a tumor-bearing small animal model. Methods: We operated on male BD X rats (260–300 g), performing either laparoscopic (n= 9) or open colon resection (n= 9) in order to evaluate stress and immunological response to laparoscopic vs conventional colon resection. A third group (n= 9) underwent anesthesia only. Immediately before and after surgery, as well as at 1, 7, and 21 days postoperatively, a sample of 1 ml blood was taken from the retrobulbar venous plexus. Stress (corticosterone) and immune parameters (neopterine and IL-1β, IL-6) and body weight as a parameter of postoperative recovery were measured to identify short-term alterations. Long-term changes were evaluated in terms of survival time and at autopsy by measuring the tumor weight and the number of tumor infiltrated nodules (histology). Results: The analysis of variance (ANOVA) showed significant differences between the three groups over a period of 1 week (p 〈 0.001 for corticosterone, p= 0.009 for neopterine, p= 0.04 for IL-1β, p= 0.024 for IL-6). Additionally, significant differences by t-test were found between the laparoscopic (minor alteration) and conventional (major alteration) group regarding corticosterone (p= 0.0015), neopterine (p= 0.0024), IL 1-β (p= 0.033), and IL-6 (p= 0.015) at the end of the operation. One week after the operation, the body weight was different depending on the type of operative procedure: 7 days postoperatively the rats lost 8% of their body weight after open surgery but only 4.3% after laparoscopic surgery. After anesthesia only, body weight increased by ∼4.8%. The medium survival time for the lap group was 44 days, whereas it was 44.1 days for the conventional group and 46 days for the anesthesia group (ANOVA p= 0.625). The number of nodules was 13.5 in the laparoscopic group 10.5 in the open group, and 7.4 in the anesthesia group, (ANOVA p= 0.119). The tumor weight was 6.8 g in the laparoscopic group, 6.4 g in the open group, and 5.04 g in the anesthesia group (ANOVA p= 0.874). Conclusion: In a tumor-bearing small animal model, laparoscopic colon resection alters the stress and immune system less than open colon resection. This observation has no implications for the long-term results as measured by survival time and at autopsy. Therefore, laparoscopic colon resection has a short-term benefit and has no negative effect on long-term results compared to conventional operative procedures.