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  • 2000-2004  (2)
  • Key words: Ultrasonic coagulator — Thoracoscopy — Internal mammary artery harvest  (1)
  • Nonhuman primates  (1)
Materialart
Erscheinungszeitraum
  • 2000-2004  (2)
Jahr
Schlagwörter
  • 1
    ISSN: 1432-2277
    Schlagwort(e): Key words Metalloproteinases ; Coronary arteriosclerosis ; Cardiac allografts ; Nonhuman primates
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) are important in any process of tissue remodeling. However, there is no report evaluating their expression in cardiac allografts in human or nonhuman primates. Heterotopic cardiac transplantation was performed on Japanese monkeys. Subjects were treated with chimeric anti-human lymphocyte function-associated antigen-1 monoclonal antibody for 2 weeks. Heart grafts were harvested at days 1–95 (n = 7). Native monkey hearts were used as controls (n = 2). We examined expression of MMP-2, MMP-9, TIMP-1, and TIMP-2 using immunohistochemistry and in situ reverse transcriptase polymerase chain reaction (RT-PCR). In the myocardium, the expression of MMP-2 was increased in the spindle-shaped cells of acutely rejected myocardial interstitium and prior to the presence of mononuclear cell infiltration at days 1–41. TIMP-1 and 2 expression was enhanced in association with the progression of fibrosis at days 40–95. In the coronary arteries of chronically rejected allografts, enhanced MMP and decreased TIMP expression was observed in both thickened intima and media at days 40–95. The medial MMP mRNA expression was observed before the development of intimal thickening occurred at days 7–28. MMPs are critical for the progression of acute and chronic rejection, and TIMP predominance plays important roles in fibrosis in association with acute rejection. Expression of MMPs and TIMPs is a sensitive indicator of acute and chronic cardiac rejection.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Surgical endoscopy and other interventional techniques 14 (2000), S. 82-85 
    ISSN: 1432-2218
    Schlagwort(e): Key words: Ultrasonic coagulator — Thoracoscopy — Internal mammary artery harvest
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Background: The Harmonic Scalpel (HS; Ethicon Endo-Surgery, Cincinnati, OH, USA) is an ultrasonic coagulator that generates less heat than electrocautery. We compared canine internal mammary arteries (IMAs) harvested using the HS or electrocautery and reviewed the early clinical outcome after thoracoscopic IMA takedown with the HS. Methods: Using HS and bipolar electrocautery (BE), 51 and 49 IMA branches, respectively, in six mongrel dogs were divided. The divided branches were subjected to a pressure tolerance test. The impact of coagulation on the main trunk was investigated microscopically. Thoracoscopic IMA harvesting with the HS was performed in 41 patients. Seven human IMA segments including 10 branches were examined microscopically. Postoperative angiography of 40 IMA grafts (97.6%) was performed. Results: All the IMA branches withstood intraluminal pressures up to 250 mmHg. With BE, coagulation reached the adventitia of the main trunk in six segments (12.2%), but the HS caused no injury. Thoracoscopic IMA harvesting with excellent hemostasis was achieved using only the HS in all 41 patients. The main trunks of the human IMA segments were microscopically intact, and postoperative angiography demonstrated that the IMA grafts were intact. Conclusion: The HS appears to be a reliable coagulator for thoracoscopic IMA harvesting with minimal damage.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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