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  • Chrysanthemum morifolium  (1)
  • Key words Agrobacterium  (1)
  • 1
    ISSN: 1432-2242
    Keywords: Key words Agrobacterium ; Chitinase ; T-DNA ; Sheath blight ; Rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A concise T-DNA element was engineered containing the rice class-I chitinase gene expressed under the control of CaMV35S and the hygromycin phosphotransferase gene (hph) as a selectable marker. The binary plasmid vector pNO1 with the T-DNA element containing these genes of interest was mobilized to Agrobacterium tumefaciens strain LBA4404 to act as an efficient donor of T-DNA in the transformation of three different indica rice cultivars from different ecosystems. Many morphologically normal, fertile transgenic plants from these rice cultivars were generated after Agrobacterium-mediated transformation using 3-week-old scutella calli as initial explants. Stable integration, inheritance and expression of the chimeric chitinase gene were demonstrated by Southern blot and Western blot analysis of the transformants. Bioassay data showed that transgenic plants can restrict the growth of the sheath blight pathogen Rhizoctonia solani. Bioassay results were correlated with the molecular analysis. Although we obtained similar results upon DNA-mediated transformation, this report shows the potential of the cost-effective, simple Agrobacterium system for genetic manipulation of rice cultivars with a pathogenesis-related (PR) gene.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Plant cell, tissue and organ culture 60 (2000), S. 33-38 
    ISSN: 1573-5044
    Keywords: chimera ; Chrysanthemum morifolium ; gamma irradiation ; shoot regeneration ; somatic mutation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rooted cuttings of Chrysanthemum morifolium cv. Maghi, a small flowered, late blooming cultivar, were treated with different doses of gamma rays. Somatic mutations in flower colour (light mauve, white, light yellow and dark yellow) and chlorophyll variegation in leaves were detected as chimeras in treated populations. Attempts were made to standardize a microtechnique for plant regeneration from mutated tissues of stem node, stem internode, shoot tip and ray floret. All these explants were cultured on Murashige and Skoog's medium with 3% sucrose, 0.8% agar and different concentrations and combinations of growth regulators. Plant regeneration was successful from all of the mutated tissues. Plants with chlorophyll variegation in leaves and two new flower colours (light mauve and white) were isolated in pure form with 64% and 100% efficiency of mutant recovery, respectively. Attempts are being made to use this technique to establish new varieties from chimeric tissues to meet the increasing demand of the floriculture trade.
    Type of Medium: Electronic Resource
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