ISSN:
1432-2242
Keywords:
Key words Hordeum vulgare
;
Hordeum bulbosum
;
Leaf rust resistance
;
Gene introgression
;
In situ hybridization
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Several disease-resistant recombinants between barley (Hordeum vulgare) and bulbous barley grass (H. bulbosum) have been obtained in recent years, but the process of characterization is often laborious and time-consuming. In order to improve the identification and chromosomal location of introgressed chromatin from H. bulbosum into the barley genome, we employed sequential genomic in situ hybridization (GISH) and fluorescence in situ hybridization (FISH). GISH enabled us to establish that an introgression was present in the disease-resistant recombinant line, and the subsequent use of FISH, with a short oligonucleotide sequence as probe, allowed us to locate the introgression on the long arm of barley chromosome 2H. These data were confirmed using RFLP probes that hybridize to barley chromosome 2HL.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/PL00002904
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