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  • 1
    Electronic Resource
    Electronic Resource
    An intervarietal molecular marker map in Triticum aestivum L. Em. Thell. and comparison with a map from a wide cross (1997)
    Springer
    Theoretical and applied genetics 94 (1997), S. 367-377 
    Details
    ISSN: 1432-2242
    Keywords: Key words Triticum aestivum ; Doubled haploids   ; Intervarietal map ; Distortion segregation ; Genetic map comparison
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  An intervarietal molecular marker map covering most of the nuclear genome was developed in Triticum aestivum. One hundred and six androgenetic-derived doubled haploid lines obtained from the F1 between monosomics of ‘Chinese Spring’ and ‘Courtot’ were analysed for genetic mapping. The map covered 18 of the 21 chromosomes with an identical distribution of markers in the A and B genome, and only small segments of the D genome. Distorted markers were mapped using Bailey’s 2-point method and revealed skewed regions on 1A, 1DS, 2A, 2B, 4AS and 6B. Comparison with a wide cross [‘Opata’×Synthetic hexaploid (T. tauschii/‘Altar 84’)] showed colinearity for markers on homologous chromosomes, but revealed a large proportion (25%) of markers mapped on non-homoeologous chromosomes, i. e. heterologous markers. The origin of the material and distortion segregation are discussed with particular emphasis on investigations of D-genome markers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050425
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  • 2
    Electronic Resource
    Electronic Resource
    Location and mapping of the powdery mildew resistance gene MlRE and detection of a resistance QTL by bulked segregant analysis (BSA) with microsatellites in wheat (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 1217-1224 
    Details
    ISSN: 1432-2242
    Keywords: Key words Triticum aestivum ; Blumeria graminis f. sp. tritici ; QTL mapping ; Molecular markers ; Disease resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Powdery mildew (Blumeria graminis f. sp. tritici) is one of the most damaging diseases of wheat (Triticum aestivum). The objective of this study was to locate and map a recently identified powdery mildew resistance gene, MlRE, carried by the resistant line RE714 using microsatellites uniformly distributed among the whole genome together with a bulked segregant analysis (BSA). The bulks consisted of individuals with an extreme phenotype taken from a population of 140 F3 families issued from the cross between RE714 (resistant) and Hardi (susceptible). The population had been tested with three powdery mildew isolates at the seedling stage. Qualitative interpretation of the resistance tests located the MlRE gene on the distal part of the long arm of chromosome 6A. A subsequent quantitative interpretation of the resistance permitted us to detect another resistance factor on a linkage group assigned to chromosome 5D, which was constructed with microsatellites for which a polymorphism of intensity between bulks was observed. This quantitative trait locus (QTL) explained 16.8– 25.34% of the total variation. An interaction between both the resistant factor (MlRE and the QTL) was found for only one of the isolates tested. This study shows the advantage of making a quantitative interpretation of resistant tests and that the use of microsatellites combined with BSA is a powerful strategy to locate resistance genes in wheat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051427
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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