ISSN:
1432-203X
Keywords:
Key words Carrot
;
Somatic embryogenesis
;
Conditioned medium
;
High-cell-density culture
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Carrot somatic embryogenesis was strongly inhibited in high-cell-density cultures. This inhibition was not caused by depletion of nutrients or physical damage but by factor(s) released into the culture medium from cells during culture. A conditioned medium prepared by eliminating cells after high-cell-density culture inhibited somatic embryogenesis. The degree of inhibition increased with the amount of conditioned medium. A dialysis experiment revealed that the molecular weight of the inhibiting factor(s) was below 3,500. We also found that the conditioned medium contained a high-molecular-weight factor(s), which stimulated somatic embryogenesis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050522
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