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  • 1
    Electronic Resource
    Electronic Resource
    Characterization of the Saccharomyces cerevisiae FCY1 gene encoding cytosine deaminase and its homologue FCA1 of Candida albicans (1997)
    Springer
    Current genetics 31 (1997), S. 1-6 
    Details
    ISSN: 1432-0983
    Keywords: Key words Pyrimidine salvage pathway  ;  Cytosine deaminase  ;  FCY1  ;  FCA1
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract By functional complementation of a fcy1 null mutant of Saccharomyces cerevisiae, we have cloned and characterized the FCY1 gene, encoding cytosine deaminase in Saccharomyces cerevisiae, and its homologue FCA1, encoding cytosine deaminase in Candida albicans. Disruption of FCY1 resulted in high resistance to 5-fluorocytosine (10−2 M) and in total loss of cytosine deaminase activity. By contrast the transformation by FCY1 or FCA1 of the haploid FCY1-disrupted host strain restored sensitivity to 5-fluorocytosine and allowed growth on cytosine, as a source of pyrimidine, or ammonium. FCA1 as opposed to FCY1 contains an intron. FCA1 and FCY1 encode respectively 150- and 158- residue proteins of 60% identity. Both Fcy1p and Fca1p share common motifs with cytidine and CMP deaminases, but homology with cytosine deaminase of E. coli could not be detected.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050169
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    New insights into the pyrimidine salvage pathway of Saccharomyces cerevisiae: requirement of six genes for cytidine metabolism (1999)
    Springer
    Current genetics 36 (1999), S. 130-136 
    Details
    ISSN: 1432-0983
    Keywords: Key words Pyrimidine salvage pathway ; Cytidine deaminase ; Cytidine metabolism ; CDD1 ; Uridine/cytidine kinase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cytidine metabolism in the yeast Saccharomyces cerevisiae was analyzed by genetic and biochemical approaches. Disruption of a unique ORF (Genbank accession No. U 20865) bearing homology with eucaryotic or bacterial cytidine deaminases abolished cytidine deaminase activity and resulted in 5-fluorocytidine resistance. The gene encoding cytidine deaminase will be referred to as CDD1 (Genbank accession number AF080089). The ability to isolate mutants resistant to 5-fluorocytidine which mapped to five other loci demonstrated the existence of a complex cytidine metabolic network. Deciphering this network revealed several original features: (1) cytidine entry is mediated by the purine-cytosine transporter (Fcy2p), (2) cytidine is cleaved into cytosine by the uridine nucleosidase (Urh1p), (3) cytidine is phosphorylated into CMP by the uridine kinase (Urk1p), (4) a block in cytosine deaminase (Fcy1p), but not in cytidine deaminase (Cdd1p), constitutes a limiting step in cytidine utilisation as a UMP precursor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050482
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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