Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Culturability and survival of an extreme thermophile isolated from deep-sea hydrothermal vents (1996)
    Springer
    Archives of microbiology 166 (1996), S. 64-67 
    Details
    ISSN: 1432-072X
    Keywords: Key wordsThermococcus peptonophilus ; Deep-sea ; Hydrothermal vents ; Survival ; Extreme thermophiles ; Culturability ; Active cells ; Mortality ; Dead cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The culturability of a strictly anaerobic, extremely thermophilic archaeon, Thermococcus peptonophilus (optimal growth temperature: 85° C), was studied during survival stages at various temperatures (98, 85, 70, and 4° C). Total cell number (determined by DAPI staining), active cells (rhodamine-stained cells), and culturable cells (using most-probable-number) were counted over time. The number of culturable cells decreased under each condition tested. The total number of cells significantly decreased only at temperatures close to the maximum for growth (98° C); at this temperature, the cells spontaneously lysed. Our results suggested that survival at 4° C in oxygenated waters might be a mechanism for the dispersion of extreme thermophiles in the ocean. In addition, we proved the existence of T. peptonophilus cells in several physiological states: culturable cells, active non-culturable cells, inactive non-culturable cells, and dead cells. Cell death was caused by cellular lysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050356
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Pressure and temperature effects on growth and viability of the hyperthermophilic archaeon Thermococcus peptonophilus (1997)
    Springer
    Archives of microbiology 168 (1997), S. 1-7 
    Details
    ISSN: 1432-072X
    Keywords: Key wordsThermococcus peptonophilus ; Extreme thermophiles ; Large-scale cultivation ; Hydrostatic ; pressure ; Barophiles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We studied the effects of high temperatures and elevated hydrostatic pressures on the physiological behavior and viability of the extremely thermophilic deep-sea archaeon Thermococcus peptonophilus. Maximal growth rates were observed at 30 and 45 MPa although no significant increases in cell yields were detected. Growth at 60 MPa was slower. The optimal growth temperature shifted from 85° C at 30 MPa to 90–95° C at 45 MPa. Cell viability during the stationary phase was also enhanced under high pressure. A trend towards barophily at pressures greater than those encountered in situ at the sea floor was demonstrated at increasing growth temperatures. The viability of cells during starvation, at high temperature (90, 95° C), and at low temperature (10° C) was enhanced at 30 and 45 MPa as compared to atmospheric pressure. These results show that the extremely thermophilic archaeon T. peptonophilus is a barophile.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050462
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...