ISSN:
1432-0428
Keywords:
Keywords Glucose-regulated gene
;
cDNA subtraction
;
gene expression
;
polymerase chain reaction
;
expressed sequence tag.
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Various genes are differentially expressed in cells during cell differentiation, development, aging, and in pathological conditions. To identify and isolate the genes that are specifically and differentially expressed in cells, we established a ligation-mediated polymerase chain reaction (PCR)-based method for cDNA subtraction. As this method is PCR-based, when target genes are expressed at high levels relative to the driver (a control pool for subtraction), even a small amount of target genes can be amplified. By this newly developed PCR-based subtraction method, a set of genes regulated by glucose were identified in a mouse insulinoma cell line. This PCR-based and non-radioactive subtraction method will be a powerful tool for identification of novel genes, specifically and differentally expressed in cells. [Diabetologia (1996) 39: 1293–1298]
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s001250050572
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