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  • 1
    ISSN: 1437-7799
    Keywords: Key words Mouse ; Uroguanylin ; Kidney ; Guanosine 3′,5′-Cyclic monophosphate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background. We constructed the expression profile of proximal tubules, which is a database of 3′-directed partial cDNA sequences randomly collected from mouse proximal tubules. By comparing lists with those of various tissues, genes unique to each tissue can be identified. Methods. One of the sequences, GS4068, corresponding to a tissue-specific gene found only in mouse renal proximal tubules, was cloned and identified as mouse uroguanylin. Northern blot analyses were performed using mRNA isolated from the kidney and intestine of dehydrated and NaCl-loaded mice. In situ hybridization was done to localize its expression in the kidney. Results. In situ hybridization demonstrated that it was located around the corticomedullary junction of the kidney. Seventy-two h of dehydration induced the mRNA expression in the kidney but not in the intestine. Acute NaCl loading, however, did not induce mRNA in the kidney or in intestine. Conclusion. Mouse uroguanylin was localized presumably in the proximal tubules of the kidney. Its mRNA in the kidney was induced by 72-h dehydration, but not by acute NaCl loading.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-069X
    Keywords: Atopic dermatitis ; Eosinophils ; FcεRI ; CD23 ; IgE
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Expression of the high affinity IgE receptor (FcεRI) on eosinophils has recently been reported. This led us to evaluate FcεRI expression on eosinophils in atopic dermatitis (AD). Double immunofluorescence stainings with an anti-FcεRI monoclonal antibody (mAb) and a polyclonal antieosinophil cationic protein (ECP) antibody were performed on lesional biopsy specimens from patients with AD and from patients with bullous pemphigoid (BP) as controls. In AD and BP lesions, 77% and 70% of eosinophils expressed FcεRI, respectively. However, the intensity of FcεRI staining in AD was much stronger than in BP, suggesting upregulation of FcεRI expression on eosinophils in AD. In addition, the eosinophils infiltrating AD lesions were stained strongly with anti-CD23 mAb and anti-IgE antibody. At the sites of mite patch testing in AD, FcεRI-, CD23- and IgE-positive eosinophils were observed to the same degree as in the lesions, and a considerable number of mite antigen-bearing eosinophils were detected. FcεRI and CD23 were both upregulated on the skin-infiltrating eosinophils in AD and bound IgE molecules.
    Type of Medium: Electronic Resource
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