ISSN:
1612-1112
Schlagwort(e):
HPLC
;
Reversed-phase
;
L-DOPA, purity of
;
L-leucine derivatives
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Chemie und Pharmazie
Notizen:
Summary The separation and quantitation of D-DOPA as an impurity in L-DOPA is described. The method involves formation of the diastereomeric derivatives of DOPA with tert-butoxycarbonyl-L-leucine hydroxysuccinimide ester. Separation of the diastereomers was performed by reversed-phase liquid chromatography on LiChrosorb RP-18 and acetonitrile-phosphate buffer with addition of N,N-dimethyloctylamine as the mobile phase. The reproducibility of the method was 5.9% (relative standard deviation) at D-DOPA levels of 0.5% in L-DOPA drug substance.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF02265633
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