ISSN:
1432-1335
Keywords:
Key words IL-12
;
IL-2
;
LAK
;
CTL
;
Cancer
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract In this study, we evaluated the ability of combination regimens of interleukin-12 (IL-12) and interleukin-2 (IL-2) to induce effective killer cells against human tumors in vitro, in peripheral blood lymphocytes (PBL) from 15 cancer patients and mixed lymphocyte/tumor culture (MLTC) cells from 16 cancer patients, and carried out a phenotypic analysis of the cells responsible for the lysis of the human tumors. The freshly prepared PBL were cultivated with IL-2 alone or IL-12/IL-2 for 10 days [lymphokine-activated killer (LAK) cell generation system]. The MLTC cells (PBL cultured with mitomycin-C-treated allogeneic G-415 tumor cells for 3 days) were further cultivated with IL-2 or IL-12/IL-2 for 7 days [cytotoxic T lymphocytes (CTL) generation system]. The cytolytic activities of the lymphoid cells cultivated with IL-12/IL-2 were significantly augmented in both the LAK and CTL generation systems, as compared with those of cells treated with IL-2 alone. In the LAK generation system, the cytolytic activities of the cells cultivated with IL-12/IL-2 were significantly decreased by the method of negative selection of CD11b+ or CD56+ cells using immunomagnetic beads. The CD8+-depleted cells showed a slight decrease of activity. The killer cell activities of the CD4+-depleted cells remained unchanged. In the CTL generation system, the activity was markedly reduced by the elimination of the CD8+ or CD11b+ or CD56+ cells. The combined data suggested that IL-12/IL-2-induced killer effector cells in the LAK generation system were mainly of the natural killer (NK) type, comprising CD8−CD11b+, CD8− CD16b+, CD3−CD56+, and partly possible CD8+ CD11b− T cells. CD8+CD11b− T cells mixed with cells of the NK type, comprising CD8−CD11b+, CD8− CD16b+ and CD3−CD56+ cells, were the population of killer effector cells induced by IL-12/IL-2 in the CTL generation system.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s004320050091
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