ISSN:
1573-5044
Keywords:
in vitro regeneration
;
organogenesis
;
clonal propagation
;
essential oils
;
Labiatae
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Various explants of Minthostachys andina (Brett.) were evaluated for their morphogenic potential under in vitro culture conditions. Axillary buds derived from 2 year-old plants grown in MS-medium supplemented with 4.4 or 8.8 µM BA and 0.054 µM NAA, initiated shoot growth and new shoot formation. Under subculture in NN medium, shoots were rooted in the presence of NAA (1.6, 2.7 or 5.3 µM) alone or in combination with IBA (9.8 µM), and the regenerated plantlets were later acclimatised in the greenhouse. Also, polynodal segments from seedlings initiated multiple shoots and plantlets when initially cultured in presence of NN-liquid salt medium supplemented with 2.2-17.7 µM BA or 4.5-13.6 M TDZ in combination with different auxin-like growth regulators and after a final transfer for root initiation. The same types of responses were found in hypocotyl and leaf explants, which produced adventitious shoots in the presence of TDZ. The use of antioxidants helped to prevent browning and favoured organogenesis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1005840706215
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