ISSN:
1040-0397
Keywords:
Thin-layer cross-flow amperometric cell
;
Osmium redox polymer
;
Oxidase electrode
;
Peroxidase electrode
;
Glucose
;
Lactate
;
Acetylcholine
;
Choline
;
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
Notes:
Thin-layer cross-flow amperometric detectors with enzyme modified working electrodes were developed for the determination of oxidase substrates in flowing streams. The enzyme was covalently immobilized in an osmium redox polymer film on the electrode surface. Electrodes modified with glucose oxidase or lactate oxidase were prepared for the continuous monitoring of glucose or lactate. A peroxidase electrode coupled with oxidase enzyme reactors was also explored for the determination of glucose, lactate, and acetylcholine/choline in continuous monitoring systems or following liquid chromatography. The effects of interferences, oxygen, mass transfer limiting membrane, and perfusate composition on electrode performance are discussed.
Additional Material:
6 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/elan.1140080803
Permalink
