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Growth and differentiation of human keratinocytes cultured on eye lens capsules (1985)

Lenoir, M. C. ; Bernard, B. A. ; Ferracin, J. ; [et al.]

Springer

Archives of dermatological research 278 (1985), S. 120-125

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ISSN: 1432-069X

Keywords: Keratinocytes ; Differentiation ; Lens capsule ; Immunofluoresence

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Summary The efficiency of the outgrowth of human epidermal and hair-follicle-sheath keratinocytes was studied using three different growth substrates: plastic, type-I collagen and bovine eye lens capsules (the Epicult system). It was shown that the eye lens capsule is the best substrate, since a higher percentage of cultures showed outgrowth, and the outgrowth of epidermal keratinocytes was much more rapid. This effect is related to the faster migration (not proliferation) of cells grown on lens capsules as compared to the two other substrates. The view that lens capsules can replace the basement membrane present in vivo was supported by the finding that two basement-membrane components, i.e., laminin and fibronectin, are present on lens capsules. It was shown that, in cultures grown on lens capsules, bullous-pemphigoid antigen is restricted to the basal layer, indicating that the differentiation of these cells is comparable to that of keratinocytes grown on irradiated, non-viable pig dermis.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00409218

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Computation of waves generated by submarine landslides (1992)

Verriere, M. ; Lenoir, M.

Chichester : Wiley-Blackwell

International Journal for Numerical Methods in Fluids 14 (1992), S. 403-421

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ISSN: 0271-2091

Keywords: Linearized surface waves ; Three-dimensional ; Fast Fourier transform ; Green function ; Finite element ; Rectilinear coast ; Engineering ; Engineering General

Source: Wiley InterScience Backfile Collection 1832-2000

Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics

Notes: Waves generated by submarine landslides are treated as three-dimensional flows of a perfect incompressible fluid. For the solution of the Cauchy-Poisson problem a time-discretization is applied which leads at each time step to a non-homogeneous free surface condition; the solution is then divided into two parts. The first part, subject to the true free surface condition, is computed in a simplified domain with constant depth. The second part involves a homogeneous free surface condition, a corrected bottom condition and the true bathymetry. In the case of constant depth, unconditional stability of the time discretization is derived. In the case of variable depth, mass and energy conservation is derived. Numerical results are presented. Comparison is made with other methods for the generation of axisymmetric waves. The transient propagation along a rectilinear coast is studied, including a comparison between two different bathymetries; trapping of energy is observed.

Additional Material: 20 Ill.