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  • 1
    Electronic Resource
    Electronic Resource
    Immobilized glucose dehydrogenase for cofactor regeneration in heme-catalyzed demethylation and hydroxylation reactions (1986)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 6 (1986), S. 265-271 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Glucose dehydrogenase (E.C. 1.1.1.47) from B. megaterium M 1286 was immobilized together with mutarotase (E.C. 5.1.3.3) on several organic carriers and by different methods. The storage stability of the enzyme at pH-values 〉 6 is slightly improved by immobilization and the pH-optimum is shifted from 8.3 to 8.0. Kinetic constants of the immobilized enzyme are: K′M(NAD+) = 5.36 × 10-4 mol/l K′M(glucose) = 3.76 · 10-2 mol/l and V′max = 5.54 · 10-5 mol/(l min g carrier) for the most active preparation (2.16 mg enzyme/g carrier).In reactor experiments the immobilized glucose dehydrogenase was used with glucose to regenerate NADPH in NADPH-dependent iron-III-protoporphyrin-IX-imidazole catalyzed hydroxylation and demethylation of model substrates of cytochrome P-450.The advantages of the coupling of both reactions with cofactor recycling are shown and discussed.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370060314
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Konservierung von Mikroorganismen in Polyvinylalkohol (1989)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 9 (1989), S. 197-200 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: A method is described for storage of microbial strains. The microorganisms are mixed with a 10% solution of polyvinylalcohol and stored at 4°C. It is demonstrated that most microbial strains studied remained uncharged were stable for 1 year.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370090222
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Adenylate cyclase in membrane fractions of RIN-A2-cells: Studies with forskolin, NaF, GppNHp and NEM (1988)
    New York, NY [u.a.] : Wiley-Blackwell
    Cell Biochemistry and Function 6 (1988), S. 131-135 
    Details
    ISSN: 0263-6484
    Keywords: Adenylate cyclase ; insulin-producing tumour cells ; N-proteins ; forskolin ; GppNHp ; thiols ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In crude membrane fractions of rat pancreatic islets and of RIN-A2-cells, forskolin and NaF stimulated adenylate cyclase activity. Basal and stimulated enzyme activity was approximately 3 to 6 fold higher in membranes of RIN-A2-cells than in membranes of islet cells. In RIN-A2-cells GppNHp and NEM inhibited forskolin-stimulated cyclase system of RIN-A2-cells contains inhibitory and stimulatory N-proteins and that there are critical thiols related to Ni, Ns and/or the catalytic unit. Thus, membrane fractions of RIN-A2-cells may be an appropriate model for studies on the adenylate cyclase system of insulin-producing cells.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/cbf.290060208
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    Paper (German National Licenses)
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