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  • Life and Medical Sciences  (19)
  • CCAATT enhancer binding protein  (2)
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Journal of molecular medicine 74 (1996), S. 347-352 
    ISSN: 1432-1440
    Schlagwort(e): Key words Adipocytes ; CCAATT enhancer binding protein ; Gene expression ; Nuclear receptors ; Peroxisome proliferator activated receptors
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract  Differentiation of adipogenic precursor cells into mature adipocytes is a complex phenomenon, characterized by an ordered expression of adipocyte-specific genes, triggered by a set of interacting transcription factors. The most important transcription factors involved in this process are the γ form of peroxisome proliferator activated receptors (PPARγ) and the various members of the CCAAT enhancer binding proteins (α, β, and δ). In addition to PPARγ and these enhancer binding proteins, several other transcription factors, including ADD-1 (SRE-BP), HMGI-C, are involved in regulating this process. Altered activity and/or expression of these transcription factors, will induce the expression of target genes in the differentiating cells, ultimately resulting in the phenotypical characteristics of the adipocytes. It is speculated that modulation of these transcription factors by either pharmacological or dietary manipulations might influence adipocyte differentiation and prove beneficial in the prevention and treatment of obesity.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Journal of molecular medicine 74 (1996), S. 347-352 
    ISSN: 1432-1440
    Schlagwort(e): Adipocytes ; CCAATT enhancer binding protein ; Gene expression ; Nuclear receptors ; Peroxisome proliferator activated receptors
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Differentiation of adipogenic precursor cells into mature adipocytes is a complex phenomenon, characterized by an ordered expression of adipocyte-specific genes, triggered by a set of interacting transcription factors. The most important transcription factors involved in this process are the γ form of peroxisome proliferator activated receptors (PPARγ) and the various members of the CCAAT enhancer binding proteins (α, β, and δ). In addition to PPARγ and these enhancer binding proteins, several other transcription factors, including ADD-1 (SRE-BP), HMGI-C, are involved in regulating this process. Altered activity and/or expression of these transcription factors, will induce the expression of target genes in the differentiating cells, ultimately resulting in the phenotypical characteristics of the adipocytes. It is speculated that modulation of these transcription factors by either pharmacological or dietary manipulations might influence adipocyte differentiation and prove beneficial in the prevention and treatment of obesity.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    ISSN: 0362-2525
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: Cytoarchitecture of the choroid plexus of the third ventricle and the paraphysis was investigated in some lower vertebrates to compare the histologic characteristics of these organs. Both epithelia are similar in appearance in the same class. Minor microscopic variations exist in the different classes of vertebrates, but do not provide a fundamental distinction between the two organs. The epithelia, moreover, have similar staining properties, contain mucicarmine- and PAS-reactive materials, and are derived from a common neuroepithelium. Tubules are identified in the choroid plexus and in the paraphysis; all are similarly formed by simple folding of epithelium on the surface into the stroma. The paraphyses in all vertebrates studied contain villi similar to those seen in the choroid plexus. Cilia are identified in both choroidal and paraphyseal epithelia, and are not an indication of degree of epithelial differentiation. Many types of epithelium are noted in both organs during histologic differentiation as well as in the mature stage. Functionally, the choroid plexus is active in both secretion and absorption. Accumulation of particulate material within the epithelial cytoplasm may indicate phagocytic as well as absorptive activity of cells. Based on a common neuroepithelial origin and similar histochemical properties, we conclude that the paraphysis is a modified choroid plexus. The velum transversum is an arbitrary boundary between diencephalon and telencephalon, and is itself formed of choroid plexus. The medial telencephalic ventricle is the rostral portion of the third ventricle. All neuroepithelial infoldings at the rostral end of the diencephalic roof including the velum transversum are intraventricular choroid plexuses; the neuroepithelial outpouchings in this region are the extraventricular choroid plexuses (paraphysis) of the diencephalon.
    Materialart: Digitale Medien
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  • 4
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 113 (1982), S. 261-266 
    ISSN: 0021-9541
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: In previous experiments (Grotendorst et al, 1981), we showed that platelet-derived growth factor promotes the migration of smooth muscle cells in vitro. Using a “checkerboard” analysis, we now establish that platelet-derived growth factor (PDGF) acts as a true chemoattractant for cultured aortic smooth muscle cells. Other growth factors such as epidermal growth factor, fibroblast growth factor, and insulin are not chemoattractants. The chemotactic response occurs before the initiation of DNA synthesis and is not affected by inhibition of DNA synthesis. Chemotaxis occurs at levels of PDGF lower than required for mitogenesis. RNA and protein synthesis are required for the chemotactic response. As found previously in bacteria and leucocytes, we find that methylation reactions are required for the chemotactic response. The possibility is discussed that PDGF acts in vivo at sites of vascular injury to attract smooth muscle cells from the medial layer to the luminal surface, and is involved in the early stages of the formation of atherosclerotic plaques.
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
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  • 5
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 121 (1984), S. 139-149 
    ISSN: 0021-9541
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: In cells transformed by avian sarcoma viruses or stimulated by growth factors, certain polypeptides become phosphorylated at tyrosine residues. It is not known if these cellular polypeptides are phosphorylated directly by the tyrosine-kinase activities which are associated with the viral transforming proteins and with growth factor receptors. It is also not clear if phosphorylation of these polypeptides is required for viral transformation or the response to growth factors. We describe here some observations which bear on these questions and discuss possible future approaches.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
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  • 6
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Cellular Physiology 134 (1988), S. 287-291 
    ISSN: 0021-9541
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: Laminin is a basement membrane-specific glycoprotein that promotes cell adhesion, proliferation, differentiation, and tumor cell migration. Synthetic peptides from the amino acid sequence deduced from a cDNA clone of the B1 chain of laminin were tested for their ability to promote the migration of B16F10 melanoma cells. A peptide, CDPGYIGSR, that is able to mediate epithelial cell attachment to laminin was found to promote migration, and the constituent pentapeptide YIGSR was also active but to a lesser degree. This nine-amino acid peptide blocked migration of melanoma cells to laminin but had no effect on migration to fibronectin. These data suggest that the cell-binding site and migration site on laminin share a common sequence that is unique to laminin.
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
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  • 7
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 231 (1991), S. 538-547 
    ISSN: 0003-276X
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin
    Notizen: We have combined injections of Fast Blue with lesions of the rubrospinal tract rostral and contralateral to them to determine if an ipsilateral rubrospinal projection exists in adult or developing opossums and, if so, to characterize the neurons giving rise to it. Although the results indicate that some rubral neurons project ipsilaterally, they are very few in number. Using quantitative and image analysis techniques, we have shown that 0.6% of the rubral neurons that project to the lumbar cord in adult opossums do so ipsilaterally and that such neurons are comparable in location and size to those that project contralaterally. Similar results were obtained in developing opossums. Our results are discussed in light of rubrospinal development and ongoing experiments related to rubrospinal plasticity.
    Zusätzliches Material: 7 Ill.
    Materialart: Digitale Medien
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  • 8
    ISSN: 0002-9106
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin
    Notizen: Midbrains of ten young adult rats were sectioned serially in three planes to study the detailed cytoarchitecture of the substantia nigra. These neurons measure 8 to 17 μ in diameter. In comparison, the largest neurons in the midbrain measure 20 to 40 μ; hence, neurons are designated according to size as follows: those measuring 18 to 40 μ, large; 12 to 17 μ, medium; and 8 to 11 μ, small. The substantia nigra of the rat contains non-pigmented neurons in three divisions: pars reticulata, pars compacta, and pars lateralis. The pars reticulata is the largest division and has the longest rostrocaudal extent. It contains neurons of small size medially and of medium size laterally. This finding correlates with described differences in connections. Three-dimensionally, the pars reticulata is an oblate spheroid with the long axis in the rostrocaudal plane. The pars compacta is a triangular layer dorsal to reticulata. It is subdivided into rostral and caudal portions at the level of the fibers of the basal optic root. Neurons in both subdivisions are medium in size, but cells rostrally are darker than those caudally. The pars lateralis is a column of neurons with its long axis located in the rostrocaudal plane. It lies dorsolateral to the pars reticulata and the pars compacta, with occasional connections to both by cellular strands. The pars lateralis has rostral and caudal portions; neurons rostrally are medium in size and round; caudally, however, neurons are variable in size, dark, stellate or fusiform, and have long processes.
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
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  • 9
    ISSN: 0002-9106
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin
    Zusätzliches Material: 2 Ill.
    Materialart: Digitale Medien
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  • 10
    ISSN: 0002-9106
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin
    Notizen: Four stages of development can be recognized in the histogenesis of the human telencephalic choroid plexus. Division into stages is based on alterations of outline of the plexus, characteristic appearance and biochemical content of the epithelial cells, and the components of the stroma. The stages are less distinctive in the myelencephalic and diencephalic plexus where differentiation is accomplished sooner than in the telencephalic plexus. Neuroepithelial-lined tubules are common in the choroid plexus, and are formed by folding of choroidal epithelium into the stroma. Some tubules are large enough to be designated as incipient neuroepithelial (colloid) cysts. Cysts having only connective tissue walls are also present. The choroidal epithelial cells proliferate focally, stratify and desquamate into intervillous clefts, or flow into the stroma by disruption of the epithelial basement membrane or tubular wall. These findings are confirmed by use of serial sections. The size of the developing telencephalic plexus relative to the ventricular system is small at first, then large, occupying almost the entire telencephalon, but gradually decreases during development. Glycogen is prominent in developing choroidal epithelial cells, but disappears in the mature plexus. Both epithelial and mesenchymal mucin and muco-polysaccharides are identified in the plexus. The paraphysis is re-emphasized as an extraventricular choroid plexus on the basis of a common neuroepithelial origin. It is rudimentary and inconstant in man.
    Zusätzliches Material: 1 Tab.
    Materialart: Digitale Medien
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