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  • 1
    Digitale Medien
    Digitale Medien
    Surface cap modifications in cold-treatedDrosophila melanogaster embryos (1992)
    Springer
    Cell & tissue research 270 (1992), S. 553-558 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Development, ontogenetic ; Cold exposure ; Microfilaments ; Microtubules ; Centrioles ; Drosophila melanogaster (Insecta)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary When earlyDrosophila embryos were allowed to develop at 0°C, several abnormalities in the surface cap organization were observed. Scanning electron microscopy showed that exposure to cold mainly lead to the deformation of the cortical caps and to their partial fusion with adjacent caps. The process of cellularization was presumably affected and large uncellularized areas were observed. Rhodamine-phalloidin staining showed that cap deformation was closely related to the altered microfilament distribution, which was presumably responsible for the failure of large syncytial areas to cellularize. During the process of cellularization, F-actin localization did not depend on the microtubules forming the baskets around the elongating nuclei, but was related to the subpopulation of mictotubules radiating from the centrosomes toward the plasma membrane. Only these microtubules seemed to be affected by cold treatment.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00645058
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  • 2
    Digitale Medien
    Digitale Medien
    Monoclonal antibody raised against murine IL-1 α peptide cross-reacts with a 60-kDa antigen in early Drosophila melanogaster embryo (1995)
    Springer
    Cell & tissue research 282 (1995), S. 269-275 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Mitosis ; Nuclear envelope ; Interleukin ; Development, ontogenetic ; Drosophila melanogaster (Insecta)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract Whole-mounts of Drosophila embryos were stained with the monoclonal antibody Vmp 18, raised against the peptide 199–208 of murine interleukin 1/α. Immunofluorescence observations showed that the antibody cross-reacted with an antigenic determinant that changed in localization during Drosophila development. In syncytial Drosophila embryos, the antibody recognized an epitope localized on the nuclear envelope throughout mitotic division. As cellularization occurred, the fluorescence was mainly concentrated in the apical region of the blastoderm cells. Western blot analysis of whole Drosophila embryo extracts showed that the antibody recognized a 60-kDa protein in syncytial embryos and during germ band elongation. This suggests that the 60-kDa antigen undergoes dynamic redistribution during embryogenesis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00319117
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  • 3
    Digitale Medien
    Digitale Medien
    Monoclonal antibody raised against murine IL-1 α peptide cross-reacts with a 60-kDa antigen in early Drosophila melanogaster embryo (1995)
    Springer
    Cell & tissue research 282 (1995), S. 269-275 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Key words: Mitosis ; Nuclear envelope ; Interleukin ; Development ; ontogenetic ; Drosophila melanogaster (Insecta)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract. Whole-mounts of Drosophila embryos were stained with the monoclonal antibody Vmp 18, raised against the peptide 199–208 of murine interleukin 1/α. Immunofluorescence observations showed that the antibody cross-reacted with an antigenic determinant that changed in localization during Drosophila development. In syncytial Drosophila embryos, the antibody recognized an epitope localized on the nuclear envelope throughout mitotic division. As cellularization occurred, the fluorescence was mainly concentrated in the apical region of the blastoderm cells. Western blot analysis of whole Drosophila embryo extracts showed that the antibody recognized a 60-kDa protein in syncytial embryos and during germ band elongation. This suggests that the 60-kDa antigen undergoes dynamic redistribution during embryogenesis.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004410050478
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  • 4
    Digitale Medien
    Digitale Medien
    Cleavage and membrane formation in the blastoderm of the dipteran Ceratitis capitata wied (1987)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 193 (1987), S. 305-315 
    Details
    ISSN: 0362-2525
    Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: In the Ceratitis capitata embryo, furrow formation follows the last mitosis divisions and leads to cellular blastoderm formation. This process displays several interesting features and requires the participation of bundles of microfilaments which are located at the furrow base at the onset of cytokinesis and contract synchronously, determining furrow growth. The new membranes for furrow growth seem to be largely provided by the fusion of many vesicles. Surface projections do not appear to contribute significantly to this phenomenon. At the end of cytokinesis the microfilaments are sandwiched between the plasma membrane and cisternae of endoplasmic reticulum. Subsequently the microfilaments disappear from the cytoplasmic side of the membrane but remain beneath the membranes of the connections and at the periphery of the yolk sack until gastrulation. On the basis of these observations some ultrastructural aspects of furrow formation and the role that the microfilaments may play during this process are discussed.
    Zusätzliches Material: 19 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/jmor.1051930308
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  • 5
    Digitale Medien
    Digitale Medien
    A monoclonal antibody recognizing a common antigen on Drosophila embryos and human fibroblasts (1991)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 19 (1991), S. 1-8 
    Details
    ISSN: 0886-1544
    Schlagwort(e): anti-vimentin antibody ; nuclear envelope ; centrosomes ; Drosophila early embryogenesis ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: We used a monoclonal antibody specific for vimentin from human fibroblasts to stain whole mounts of Drosophila embryos. In immunofluorescence observations this antibody cross-reacts with an antigenic determinant localized throughout mitosis at the nuclear boundary. Double fluorescence observations with the Rb188 antibody that specifically recognizes a centrosomal protein of the Drosophila embryo [Whitfield et al., 1988] showed that the anti-vimentin antibody cross-reacts with an antigen localized in the centrosomal region.
    Zusätzliches Material: 4 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/cm.970190102
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  • 6
    Digitale Medien
    Digitale Medien
    Cytoskeleton of the Drosophila egg chamber: New observations on microfilament distribution during oocyte growth (1995)
    New York, NY : Wiley-Blackwell
    Cell Motility and the Cytoskeleton 31 (1995), S. 298-306 
    Details
    ISSN: 0886-1544
    Schlagwort(e): Drosophila ; nurse cells ; oocyte ; microfilaments ; Life and Medical Sciences ; Cell & Developmental Biology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: The distribution of microfilaments in Drosophila egg chambers stained with rhodamine (Rh)-conjugated phallcidin was studied by laser scanning confocal microscopy and transmission electron microscopy. These techniques revealed new details in the pattern of microfilament localization. We observed in stage 1-3 egg chambers accumulation of filamentous actin in the oocyte cytoplasm between the ring canals connecting the oocyte with adjacent nurse cells. Starting from stages 6-7 short microfilament bundles arranged in basket-like structures were associated with the side of the ring canals facing the nurse cell cytoplasm. We also observed a dramatic decrease in the actin network associated with the cortex of the oocyte in stage 10. During stage 10B the nurse cell cytoplasm was crossed by radial actin bundles that showed a sarcomeric-like cross striation after Rh-phalloidin staining. The ring canals also did not uniformly stain but showed a punctate labeling. The implications of the actin cytoskeleton during oocyte growth are discussed. © 1995 Wiley-Liss, Inc.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/cm.970310406
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