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Digitale Medien

The selection of a stable rat hepatoma variant with concomitant increase in ploidy and permeability to glycerol (1978)

Li, C. C. ; Lin, E. C. C. ; Karnovsky, M. J. ; [weitere]

New York, NY [u.a.] : Wiley-Blackwell

Journal of Cellular Physiology 94 (1978), S. 197-203

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ISSN: 0021-9541

Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Medizin

Notizen: By repeated selection for longer survival in an isotonic solution of glycerol, a stable subline of Novikoff rat hepatoma cells has been isolated. The cells exhibit markedly increased resistances to osmotic lysis in isotonic solutions of glycerl. They are twice as large and have twice as many chromosomes as cells of the parental line. It is suggested that the osmotic stress procedure can be extended for the selection of numerous kinds of mutants and can be used as a method of analysis of membrane properties.

Zusätzliches Material: 7 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jcp.1040940209

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Digitale Medien

Glycerol transport and phosphorylation by rat hepatocytes (1983)

Li, Chien-Chung ; Lin, E. C. C.

New York, NY [u.a.] : Wiley-Blackwell

Journal of Cellular Physiology 117 (1983), S. 230-234

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ISSN: 0021-9541

Schlagwort(e): Life and Medical Sciences ; Cell & Developmental Biology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Medizin

Notizen: The entry of glycerol into isolated rat hepatocytes appears to be catalyzed by a specific carrier. At a physiological concentration of 0.1 mM, glycerol utilization is rate limited by the permeation step. Intracellular glycerol is trapped by an excess of glycerol kinase, which has a higher apparent affinity for the substrate than that of the membrane carrier. The entry of glycerol into the hepatocytes is highly sensitive to inhibition by monoacetin and cytochalasin B, but not by DL-1,2-propanediol, erythritol, D-glucose, D-galactose, D-mannose, or D-fructose.

Zusätzliches Material: 5 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/jcp.1041170214

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