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  • 1
    Electronic Resource
    Electronic Resource
    Effect of low-temperature rearing on heat shock protein synthesis and heat sensitivity in Drosophila melanogaster (1988)
    Chichester [u.a.] : Wiley-Blackwell
    Developmental Genetics 9 (1988), S. 193-201 
    Details
    ISSN: 0192-253X
    Keywords: thermotolerance ; hsp 23 ; heat shock genes ; hsr 93D ; cold rearing ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The patterns of synthesis of heat shock proteins (hsp) and heat sensitivity to elevated temperatures in larvae of Drosophila melanogaster reared since hatching at 20°C (warmreared) or at 10°C (cold-reared) were compared. The pattern of hsp synthesis in salivary glands from the cold- and warm-reared late-third-instar larvae exposed for l hr to 33°C or to 37°C was generally similar except for remarkable differences in the 23 kd hsp and a heat-inducible 14 kd polypeptide. The hsp 23 was abundantly synthesised in control as well as heat-shocked warm-reared larval salivary glands, its synthesis in heat-shocked glands being dependent on new transcription. The synthesis of hsp 23 was much less in control glands of cold-reared larvae and was not further inducible by heat shock. The 14 kd polypeptide synthesis was greater in control as well as heat-shocked salivary glands of cold-reared larvae, whereas, in the warm-reared ones, its activity was much less. The cold-reared larvae showed greater sensitivity to elevated temperature; fewer adults eclosed when the cold-reared late-third-instar larvae were exposed to 40°C for l hr and also a pretreatment at 37°C for l hr was less effective in stopping the killing effect of a subsequent 40°C heat shock in cold-reared than in warmA-reared larvae. The greater thermosensitivity of the cold-reared larvae may be correlated with the altered patterns of heat shock gene transcription and translation in cold-reared larvae.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/dvg.1020090305
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Artifacts introduced by ion milling in Al-Li-Cu alloys (1988)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 8 (1988), S. 355-361 
    Details
    ISSN: 0741-0581
    Keywords: Electropolishing ; TEM ; δ′ ; T1 and θ′ precipitates ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Ion milling is commonly used to prepare specimens for observation under transmission electron microscope (TEM). This technique sometimes introduces artifacts in specimens contributing to misleading interpretation of TEM results as observed in the present investigation of Al-Li-Cu alloys. This type of alloy, in general, contains several kinds of precipitates, namely δ′ T1, and θ′. It is found that ion milling even for a short time produces drastic changes in the precipitate characterics as compared to standard electropolishing methods of specimen preparation for TEM. Careful analysis of selected area diffraction patterns and micrographs shows that after ion milling δ′ precipitates are very irregular, whereas other precipitates coarsen and they are surrounded by misfit dislocations. In situ hot-stage TEM experiments were performed to relate the microstructure to that observed in the ion-milled specimen. Results and causes of ion milling effects on the microstructure are discussed in relation to standard electropolishing techniques and in situ hot-stage experiment.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060080404
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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