ISSN:
1432-1017
Keywords:
Key words Path clamp
;
Putrescine
;
Spermidine
;
Spermine
;
Lipid-polyamine model membranes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Physics
Notes:
Abstract The tip-dip patch clamp method was used to study the effect of three polyamines, putrescine, spermidine and spermine, on bilayer lipid membrane (BLM) stability. Two kinds of mixed lipid-polyamine membranes were investigated. The poration voltage (V p), and the closed (σcl) and open (σop) state conductances for pure and polyamine-treated lipid membranes were determined by the method of current-voltage surfaces. It was demonstrated that putrescine and spermidine destabilized lipid membranes under all circumstances. BLM stabilization by spermine was observed when it was added to preformed membranes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002490050261
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