Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Integrative transformation of the yeast Yarrowia lipolytica (1985)
    Springer
    Current genetics 10 (1985), S. 49-58 
    Details
    ISSN: 1432-0983
    Keywords: Yarrowia lipolytica ; Heterologous gene expression ; Integrative transformation ; Homologous recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An EcoR1 shotgun of Yarrowia lipolytica DNA was inserted into the plasmid YIp333 which carries the LYS2 gene of S. cerevisiae. The resulting plasmid pool was transformed in both S. cerevisiae and Y. lipolytica. Whereas numerous replicating plasmids could be isolated from the S. cerevisiae Lys+ transformants, all transformants of Y. lipolytica so far analyzed were found to result from integrative transformation. This occurred at a frequency of 1 to 10 transformants per μg of input DNA. Co-transformation occurred at high frequency and resulted in tandem integration of 2 to 10 copies of the incoming DNA. Structural and segregational stability of the transforming DNA were both high.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00418493
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Evidence for the control of a mutation in lysine catabolism by the mating type in Yarrowia lipolytica (1984)
    Springer
    Current genetics 8 (1984), S. 531-536 
    Details
    ISSN: 1432-0983
    Keywords: ROAM mutation ; Yarrowia lipolytica ; Lysine catabolism ; Regulation of lysine pool
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Reversions of a mutation lye 1.5 blocking the first step of the lysine catabolic pathway were isolated. These reversions mapped inside the LYC1 locus. They exhibited an alteration of the regulation of the lysine pool after nitrogen and phosphorus starvation. This phenomenon did not appear in sexual diploids homozygous for the reversion. Diploids homozygous for the mating type were constructed by protoplast fusion. They displayed a pattern similar to that of the haploids. We conclude that the expression of this mutation is under the control of the physiological state of the mating type.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00410440
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...