ISSN:
1573-0603
Keywords:
Chemoprevention
;
Mammary alveolar lesions
;
Mammary gland
;
Organ culture
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Mouse mammary glands respond to growth promoting hormones in organ culture. In the presence of insulin, prolactin, aldostrone, and hydrocortisone, the glands exhibit extensive proliferation within 10 days of culture mimicking the mammary alveolar structures observed during pregnancy. However withdrawal of prolactin and steroids from the medium for an additional 14 days results in the disintegration of the alveolar structures resembling the mammary morphology observed during the involution stage. During the growth promoting phase if the glands are exposed to 7, 12, dimethylbenz(a)anthracene (DMBA) for 24 hours and cultured through the entire 24 days of culture period, they develop precancerous lesions. This model is highly reproducible and extensively utilized to evaluate efficacy of potential chemopreventive agents against carcinogen-induced mammary lesions.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1023/A:1009770720081
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