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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Protoplasma 151 (1989), S. 158-163 
    ISSN: 1615-6102
    Keywords: Lectins ; Mannose ; Silica deposition vesicles ; Ditylum brightwellii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The present study on a centric diatom,Ditylum brightwellii, includes two parts: detection of sugars in the silica deposition vesicle (SDV) with lectins and labeling the developing siliceous cell wall in the SDV with rhodamine 123. Cells with developing valves are treated with SDS to remove all the cytoplasmic contents, then either stained with fluorescein labeled lectins or thin-sectioned and stained with colloidal gold labeled lectins. The results show that mannose is part of the organic matrix in the SDV. Rhodamine 123, a non-toxic fluorescent laser dye, enters the cell immediately and is trapped in the SDV probably by the high reducing potential of the SDV. Silica is co-deposited with rhodamine 123 in the SDV, and the resulting valves and girdle bands become fluorescent. Implications of this study for the mechanism of silicification are discussed.
    Type of Medium: Electronic Resource
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