ISSN:
1617-4623
Keywords:
Bent DNA
;
DNA melting pattern
;
Replication origin
;
Matrix attachment site
;
Globin genes
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary The distribution of specific DNA sequence elements in a 2.9 kb HindIII fragment of chicken DNA containing the replication origin and the upstream matrix attachment site (MAR) of the α-globin gene domain was investigated. The fragment was shown to contain a CR1-type repetitive element and two stably bent DNA sequences. One of them colocalizes with the previously described MAR element and with the recognition site for a proliferating-cell-specific, DNA-binding protein. The melting pattern of a set of subfragments of the region proved to be non random. No correlation between the distribution of readily melting sequences and bent DNA was found. The possible importance of curved, low-melting and repetitive DNA sequences for the organization of the upstream boundary of the α-globin gene domain and the function of the replication origin is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00279384
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