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  • Digitale Medien  (2)
  • Microfilaments  (2)
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Protoplasma 109 (1981), S. 143-157 
    ISSN: 1615-6102
    Schlagwort(e): Acetabularia ; Microfilaments ; Microtubules ; Protoplasmic streaming
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A compilation of characteristics of the two different systems of intracellular transport inAcetabularia (Koop andKiermayer 1980 a and b) is given. The presence of microfilaments-presumably F-actin-in the cytoplasm ofAcetabularia is demonstrated by electron microscopy. The evidence for an involvement of microtubules in streaming is strengthened by the induction of birefringent vinblastine crystals in the stalk of vegetative cells. Isolated portions of cytoplasm formin vitro more than 100 μm long filopodium-like processes, which are highly birefringent. The processes show intensive immunofluorescent staining with both, anti-actin and anti-tubulin as a primary antibody. A perfusion buffer is presented, which after replacing the vacuolar sap does not lead to a change in cytoplasmic morphology or streaming pattern and velocities.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Protoplasma 102 (1980), S. 295-306 
    ISSN: 1615-6102
    Schlagwort(e): Acetabularia ; Inhibitors ; Microcinematography ; Microfilaments ; Microtubules ; Protoplasmic streaming
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The sensitivity of the dual intracellular transport system inAcetabularia mediterranea (Koop andKiermayer 1979 a) to cytochalasin B (CB, 10−5 mol), chlorisopropyl-N-phenylcarbamate (CIPC, 2.10−4 mol), and amiprophosmethyl (APM, 3.10−5 mol) has been studied by microcinematography. Vegetative cells before cap formation, 2–3 cm in length, and cells with a maximum sized cap, containing secondary nuclei, were used for the experiments. All intracellular movements ceased under the influence of CB, while in contrast to “headed streaming bands” and to the migration of the secondary nuclei, the movement of chloroplasts at “thin filaments” was found to be insensitive to Col, CIPC, and APM. All inhibitory effects of the drugs on protoplasmic streaming were completely reversible within a time of less than 10–20 minutes recovery from the drugs. The results suggest an involvement of microfilaments in all intracellular movements while, in addition, microtubules seem to be connected with the movement on “headed streaming bands”, including the migration of secondary nuclei.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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