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  • 1
    Electronic Resource
    Electronic Resource
    Direct amplification of minisatellite-region DNA with VNTR core sequences in the genus Oryza (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 942-949 
    Details
    ISSN: 1432-2242
    Keywords: Key words Oryza ; Rice ; PCR ; DNA fingerprinting ; Minisatellite
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A polymerase chain reaction (PCR) application, involving the directed amplification of minisatellite-region DNA (DAMD) with several minisatellite core sequences as primers, was used to detect genetic variation in 17 species of the genus Oryza and several rice cultivars (O. sativa L.). The electrophoretic analysis of DAMD-PCR products showed high levels of variation between different species and little variation between different cultivars of O. sativa. Polymorphisms were also found between accessions within a species, and between individual plants within an accession of several wild species. The DAMD-PCR yielded genome-specific banding patterns for the species studied. Several DAMD-PCR-generated DNA fragments were cloned and characterized. One clone was capable of detecting multiple fragments and revealed individual-specific hybridization banding patterns using genomic DNA from wild species as well as rice cultivars. A second clone detected only a single polymorphic locus, while a third clone expressed a strong genome specificity by Southern analysis. The results demonstrated that DAMD-PCR is potentially useful for species and genome identification in Oryza. The DAMD-PCR technique also allows for the isolation of informative molecular probes to be utilized in DNA fingerprinting and genome identification in rice.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050645
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Repetitive, genome-specific probes in wheat (Triticum aestivum L. em Thell) amplified with minisatellite core sequences (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 982-989 
    Details
    ISSN: 1432-2242
    Keywords: Genome-specific ; DAMD ; Minisatellite ; PCR ; Triticum ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The detection and analysis of DNA polymorphisms in crops is an essential component of marker-assisted selection and cultivar identification in plant breeding. We have explored the direct amplification of minisatellite DNA by PCR (DAMD-PCR) as a means for generating DNA probes that are useful for detecting DNA polymorphisms and DNA fingerprinting in wheat. This technique was facilitated by high-stringency PCR with known plant and animal minisatellite core sequences as primers on wheat genomic DNA. The products of DAMD-PCR from Triticum aestivum, T. durum, T. monococcum, T. speltoides and T. tauschii showed a high degree of polymorphism and the various genomes could be identified. Cloning of the DAMD-PCR products and subsequent Southern hybridization frequently revealed polymorphic probes showing a good degree of genome specificity. In addition, polymorphic, single locus, and moderately dispersed PCR products were cloned that may have a potential for DNA fingerprinting. Our experiments were limited primarily to diploid wheats and the results indicated that DAMD-PCR may isolate genome-specific probes from wild diploid wheat species that could be used to monitor genome introgression into hexaploid wheat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224102
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    PCR primed with minisatellite core sequences yields DNA fingerprinting probes in wheat (1997)
    Springer
    Theoretical and applied genetics 95 (1997), S. 276-283 
    Details
    ISSN: 1432-2242
    Keywords: Key words PCR ; Minisatellite ; DNA fingerprinting ; Wheat ; Triticale
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Four minisatellite core sequences were used as primers in a polymerase chain reaction (PCR) technique, known as the directed amplification of minisatellite-region DNA (DAMD), to detect polymorphisms in three pairs of hexaploid/tetraploid wheat cultivars. In each pair, the tetraploid cultivar (genomic formula AABB) was extracted from its corresponding hexaploid (genomic formula AABBDD) parent. Reproducible profiles of the amplified products revealed characteristic bands that were present only in the hexaploid wheats but not in their extracted tetraploids. Some polymorphisms were observed among the hexaploid cultivars. Twenty-three DAMD-PCR amplified fragments were isolated and screened as molecular probes on the genomic DNA of wild wheat species, hexaploid wheat and triticale cultivars. Subsequently, 8 of the fragments were cloned and sequenced. The DAMD-PCR clones revealed various degrees of polymorphism among different wild and cultivated wheats. Two clones yielded individual-specific DNA fingerprinting patterns which could be used for species differentiation and cultivar identification. The results demonstrated the use of DAMD-PCR as a tool for the isolation of informative molecular probes for DNA fingerprinting in wheat cultivars and species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050560
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    Paper (German National Licenses)
    Fulltext
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