ISSN:
1432-072X
Keywords:
Rhizociticines
;
Plumbemicines
;
Phosphone-oligopeptide antibiotics
;
Mode of action
;
Z-2-Amino-5-phosphone-3-pentenoic acid
;
Threonine synthase
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The rhizocticines and plumbemicines are two groups of di- and tripeptid antibiotics thought to interfere with threonine or threonine-related metabolism. Z-2-amino-5-phosphono-3-pentenoic acid, the common unusual amino acid constituent of the rhizocticines and plumbemicines, was found to irreversibly inhibit Escherichia coli threonine synthase in a time-dependent reaction that followed pseudo-first order and saturation kinetics. These data provide evidence that the toxicity of the rhizocticines and plumbemicines is due to the inhibition of threonine synthase by Z-2-amino-5-phosphone-3-pentenoic acid, which is liberated by peptidases after uptake into the target cell. Additionally, methods for the purification of threonine synthase from an overproducing E. coli strain and for the enzymatic synthesis of l-homoserine phosphate are described.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00288949
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