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  • 1
    ISSN: 1432-2048
    Keywords: Aleurone (enzyme secretion) ; α-Amylase ; Gibberellin and enzyme secretion ; Hordeum (enzymes) ; Monensin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of monensin on the secretion of α-amylase and other enzymes from the aleurone layer of barley (Hordeum vulgare L. cv. Himalaya) was studied by electrophoresis followed by fluorography and by pulse-chase and organelle-isolation experiments. Monensin markedly inhibits the secretion, but not the synthesis, of α-amylase, acid phosphatase, and at least four other proteins from the aleurone layer. Monensin treatment causes α-amylase to accumulate within the protoplast, but its effect on the different α-amylase isoenzymes is not equal. The accumulation of isoenzyme 2 is not influenced by monensin while isoenzymes 1, 3 and 4 are not secreted but rather accumulate in the cell when monensin is included in the incubation medium. The α-amylase and acid-phosphatase activities which accumulate within the aleurone cells following treatment with monensin are localized in an organelle having a buoyant density greater than that of endoplasmic reticulum and less than that of mitochondria. In pulse-chase experiments with [35S]methionine, labelled proteins accumulate in this organelle in the presence of monensin and do not appear in the incubation medium. We conclude that monensin inhibits the secretion of proteins from the barley aleurone layer by influencing their intracellular transport.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1615-6102
    Keywords: α-Amylase ; Corn (Zea mays) seedlings ; Growth ; Monensin ; Polysaccharide slime ; Secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The effect of monensin on polysaccharide slime secretion by root tips of corn (Zea mays) was studied. Various treatment times and ionophore concentrations were tested: none resulted in inhibition of slime secretion. Because monensin changes the pH of the medium, its effect was also monitored in strongly buffered media and at different pH's. Even in such media, monensin did not inhibit slime secretion. We also measured the effect of the drug after a pulse with [3H]fucose or a pulse followed by a chase. The amount of labeled slimed secreted was not altered by the ionophore. However, 10μM monensin affected the development of root tips and drastically reduced their growth. We showed that monensin inhibits the secretion of α-amylase by the scutellum of the same plantlet. The importance of the nature of the secretory compound in relation to monensin inhibition of its secretion is discussed.
    Type of Medium: Electronic Resource
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