ISSN:
1432-0878
Keywords:
Monoamines
;
Pancreatic islets
;
Parafollicular cells
;
Autoradiographic
;
Fluorescence technique
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary White albino mice were simultaneously injected with 3H-5-HTP and unlabelled DOPA or 3H-DOPA and unlabelled 5-HTP. The distribution patterns of the formed indol- and catecholamines in a) the pancreatic islets and b) the parafollicular cells were then compared by use of a combined autoradiographic-fluorescence microscopic technique. Localization in the same sections of radioactivity and fluorescence representing the respective monoamines corresponded very well for all the time intervals studied both in the pancreatic islets and in the parafollicular cells. In the pancreatic islets the monoamines were localized in all cells shortly after the injection of the precursors. The monoamines then first disappeared from the β-cells leaving the α-cells with the highest concentration of activity and fluorescence. In the thyroid the activity and fluorescence, localized in the same parafollicular cells, persisted for a longer time than in the islets.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00331203
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