ISSN:
1432-0533
Keywords:
Tissue culture
;
Human peripheral neurons
;
Myelin-associated glycoprotein
;
Immunocytochemistry
;
Morphology
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Dorsal root ganglion cells, obtained from 8–10-week human foetuses, were isolated by enzymatic procedure and grown on poly-l-lysine-coated coverslips. Most of the cultured cells showed the ultrastructural and immunological features of normal peripheral neurons. By immunocytochemistry neurons reacted with IgM antibodies with specificity for myelin-associated glycoprotein (MAG) from patients affected with IgM k gammopathy and peripheral neuropathy. The antigen was located on the plasmalemma of both perikarion and axon. We suggest that anti-MAG antibodies do not recognize neuronal MAG, but rather an epitope shared with different glycoproteins.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00688257
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