ISSN:
1612-1112
Schlagwort(e):
Preparative column liquid chromatography
;
Optimization of throughput
;
Column overloading
;
Adjustment of fraction collection
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Chemie und Pharmazie
Notizen:
Summary The criteria for the optimization of preparative chromatography are sample throughput and product purity. It is shown for column liquid chromatography that the throughput has been maximized for a given purity by column overloading and appropriate fractionation. The size and position of the fraction must be determined by chemical analysis, since overlapping peaks under overloading conditions are usually distorted compared to the peaks of the same amounts of pure compounds. With overloading of the column larger particles can be used as column packings without reduction of the separation effect.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF02262687
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